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首页> 外文期刊>Canadian Journal of Physiology and Pharmacology >Cardiac-protective effects and the possible mechanisms of alatamine during acute myocardial ischemia
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Cardiac-protective effects and the possible mechanisms of alatamine during acute myocardial ischemia

机译：急性心肌缺血中阿拉米明的心脏保护作用及其可能机制

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摘要

Alatamine is a constituent in the extract of a traditional herbal medicine Ramulus euonymi widely used for cardiac protection. However, its precise effects remain unclear. In the present study, we found that alatamine was able to reduce acute myocardial ischemia (AMI)-induced cardiac dysfunction in a rat model, as reflected by significantly restored electrocardiograms, M-mode echocardiograms, and left ventricular hemodynamics. Also, Nagar Olsen staining revealed that alatamine markedly reduced AMI-induced cardiac injury and cardiac myocyte apoptosis. TUNEL and caspase-3 activity assay showed that cardiac myocytes underwent significant apoptosis during AMI, and levels of LDH and CK-MB increased in the serum. However, such changes were significantly inhibited by pre-administration of alatamine. Furthermore, such anti-apoptotic effects of alatamine was also confirmed in a cardiac myocyte model of isoproterenol (ISO)-induced damage. Mechanistically, it was also found that alatamine improved the expression and activity of sarcoplasmic/endoplasmic reticulum Ca2+ ATPase (SERCA), which were inhibited during AMI, promoting contractility and relaxation. Meanwhile, alatamine decreased Bax and increased Bcl-2 expressions both in vivo and in vitro, therefore inhibiting cardiac myocyte apoptosis and preventing cardiac dysfunction caused by AMI at the cellular level. The present study revealed the beneficial role of alatamine in cardiac protection and highlighted it as a potential therapeutic reagent for reduction of AMI-induced cardiac injury.
机译：阿拉特明是广泛用于心脏保护的传统草药Ramulus euonymi提取物中的成分。但是，其确切效果仍不清楚。在本研究中，我们发现阿拉坦胺能够减轻大鼠模型中的急性心肌缺血（AMI）引起的心脏功能障碍，这通过明显恢复的心电图，M型超声心动图和左心室血流动力学得以反映。另外，Nagar Olsen染色显示，阿拉米明可显着减少AMI引起的心脏损伤和心肌细胞凋亡。 TUNEL和caspase-3活性测定表明，AMI期间心肌细胞发生了明显的凋亡，血清中LDH和CK-MB的水平升高。但是，这种变化被预先服用阿拉明胺所抑制。此外，在异丙基肾上腺素（ISO）诱导的损伤的心肌细胞模型中也证实了丙胺的这种抗凋亡作用。从机理上讲，还发现阿拉米明改善了肌浆/内质网Ca2 + ATPase（SERCA）的表达和活性，这些蛋白在AMI期间被抑制，促进了收缩力和松弛。同时，阿拉特明在体内和体外均降低Bax并增加Bcl-2表达，因此在细胞水平上抑制心肌细胞凋亡并预防AMI引起的心脏功能障碍。本研究揭示了阿拉明胺在心脏保护中的有益作用，并强调了它是减少AMI引起的心脏损伤的潜在治疗剂。

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