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首页> 外文期刊>Canadian Journal of Physiology and Pharmacology >Effet radiosensibilisateur de l'acide linoleique conjugue chez les cellules cancereuses du sein MCF-7 et MDA-MB-231
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Effet radiosensibilisateur de l'acide linoleique conjugue chez les cellules cancereuses du sein MCF-7 et MDA-MB-231

机译：共轭亚油酸对MCF-7和MDA-MB-231乳腺癌细胞的放射增敏作用

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摘要

Apoptotic pathways in breast cancer cells are frequently altered, reducing the efficiency of radiotherapy. Conjugated linoleic acid (CLA), known to trigger apoptosis, was tested as radiosensitizer in breast cancer cells MCF-7 and MDA-MB-231. The CLA-mix, made up of the isomers CLA-9cis 11trans and CLA-10trans 12cis, was compared to three purified isomers, i.e., the CLA-9cis 11cis, CLA-9cis 11trans, and CLA-10trans 12cis. Using the apoptotic marker YO-PRO(R)-1, the CLA-9cis 11cis at 50 micro mol/L turned out to be the best apoptotic inducer leading to a 10-fold increase in MCF-7 cells and a 2,5-fold increase in MDA-MB-231 cells, comparatively to the CLA-mix. Contrary to previous studies on colorectal and prostate cancer cells, CLA-10trans 12cis does not lead to an apoptotic response on breast cancer cell lines MCF-7 and MDA-MB-231. Our results also suggest that the main components of the CLA-mix (CLA-9cis 11trans and CLA-10trans 12cis) are not involved in the induction of apoptosis in the breast cancer cells studied. A dose of 5 Gy did not induce apoptosis in MCF-7 and MDA-MB-231 cells. The addition of CLA-9cis 11cis or CLA-mix has allowed us to observe a radiation-induced apoptosis, with the CLA-9cis 11cis being about 8-fold better than the CLA-mix. CLA-9cis 11cis turned out to be the best radiosensitizer, although the isomers CLA-9cis 11trans and CLA-10trans 12cis have also reduced the cell survival following irradiation, but using a mechanism not related to apoptosis. In conclusion, the radiosensitizing property of CLA-9cis 11cis supports its potential as an agent to improve radiotherapy against breast carcinoma.
机译：乳腺癌细胞中的凋亡途径经常被改变，从而降低了放射治疗的效率。在乳腺癌细胞MCF-7和MDA-MB-231中测试了已知会触发凋亡的共轭亚油酸（CLA）作为放射增敏剂。将由异构体CLA-9cis 11trans和CLA-10trans 12cis组成的CLA混合物与三种纯化的异构体，即CLA-9cis 11cis，CLA-9cis 11trans和CLA-10trans 12cis进行了比较。使用凋亡标记物YO-PRO（R）-1，CLA-9cis 11cis浓度为50 micro mol / L成为最佳的凋亡诱导剂，导致MCF-7细胞增加10倍，而2,5,5相对于CLA混合物，MDA-MB-231细胞的CDS倍增加。与先前关于结直肠和前列腺癌细胞的研究相反，CLA-10trans 12cis不会导致乳腺癌细胞MCF-7和MDA-MB-231发生凋亡反应。我们的结果还表明，CLA混合物的主要成分（CLA-9cis 11trans和CLA-10trans 12cis）不参与所研究的乳腺癌细胞凋亡的诱导。 5 Gy剂量未诱导MCF-7和MDA-MB-231细胞凋亡。 CLA-9cis 11cis或CLA-mix的加入使我们能够观察到辐射诱导的细胞凋亡，其中CLA-9cis 11cis比CLA-mix好约8倍。 CLA-9cis 11cis被证明是最好的放射增敏剂，尽管异构体CLA-9cis 11trans和CLA-10trans 12cis也降低了辐射后的细胞存活率，但是使用的机制与细胞凋亡无关。总之，CLA-9cis 11cis的放射增敏特性支持其作为改善针对乳腺癌的放射疗法的药剂的潜力。

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