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首页> 外文期刊>Canadian Journal of Physiology and Pharmacology >Radiation-induced peroxidation of small unilamellar vesicles of phosphatidylcholine generated by sonication.
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Radiation-induced peroxidation of small unilamellar vesicles of phosphatidylcholine generated by sonication.

机译：辐射诱导的超声作用产生的小单层磷脂酰胆碱小囊泡的过氧化。

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摘要

The present study was aimed at determining the peroxidation of model membranes constituted of liposomes of 1-palmitoyl-2-linoleoyl-sn-glycero-3-phosphocholine (PLPC) submitted to hydroxyl free radicals (generated by γ-radiolysis) attack. Liposomes of PLPC were prepared using the sonication technique, and dynamic light-scattering (DLS) measurements allowed characterization of the liposomal dispersions. Irradiation damages in sonication-generated liposomes were assessed by monitoring several oxidation products, such as conjugated dienes (by means of UV–visible spectrophotometry) and hydroperoxides (using reverse phase high-performance liquid chromatography (HPLC) associated with chemiluminescence detection). It has been shown that three different families of hydroperoxides are formed: the first one (at low radiation doses) results from HO. attack on the linoleyl chain of PLPC, giving phosphatidylcholine hydroperoxides possessing a conjugated dienic structure; the two others (at high radiationdoses) are obtained by the secondary HO. attack on the primary hydroperoxide family. The quantification of these products associated with the comparison of their radiation-dose-dependent formation has provided valuable information concerning the mechanisms of their formation. Analysis by HPLC – mass spectrometry has confirmed the presence of hydroperoxides and underlined various other products, like chain-shortened fragments and oxygenated derivatives of polyunsaturated sn-2 fatty acyl chain residues. Structural assignment proposals of some oxidation products have been proposed.
机译：本研究旨在确定由1-棕榈酰基-2-亚油酰基-sn-甘油-3-磷酸胆碱（PLPC）的脂质体构成的模型膜的过氧化作用，该脂质膜受到羟基自由基（由γ辐射分解产生）的攻击。 PLPC脂质体使用超声处理技术制备，动态光散射（DLS）测量可表征脂质体分散体。通过监测几种氧化产物（例如共轭二烯（通过紫外可见分光光度法）和氢过氧化物（使用与化学发光检测相关的反相高效液相色谱（HPLC）））来评估超声产生的脂质体的辐照损伤。已经证明形成了三个不同的氢过氧化物家族：第一个（在低辐射剂量下）由HO产生。攻击PLPC的亚油酰基链，得到具有共轭二烯结构的磷脂酰胆碱氢过氧化物;其他两个（在高辐射剂量下）是通过次要HO获得的。对主要氢过氧化物家族的攻击。这些产物的定量及其与辐射剂量依赖性形成的比较相关，提供了有关其形成机理的有价值的信息。通过HPLC分析–质谱法已确认存在氢过氧化物，并在其他各种产品上加下划线，例如链缩短的片段和多不饱和sn-2脂肪酰基链残基的氧化衍生物。已经提出了一些氧化产物的结构分配建议。

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