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首页> 外文期刊>Canadian Journal of Physiology and Pharmacology >Induction of apoptosis in K562 cells by jolkinolide B.
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Induction of apoptosis in K562 cells by jolkinolide B.

机译：千金藤内酯B诱导K562细胞凋亡。

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摘要

Jolkinolide B, a bioactive diterpene isolated from the roots of Euphorbia fischeriana Steud, has various biological and pharmacological properties. In this study, the cytotoxicity of highly purified jolkinolide B was tested in human chronic myeloid leukemia (K562) and 2 other cell lines (human esophageal carcinoma Eca-109 and human hepatoma HepG2). The results indicate a significant decrease in the proliferation of all the 3 cell lines when treated with jolkinolide B for 24 h; the IC50 value of cytotoxicity was 12.1 mug/mL (for K562 cells), >50.0 mug/mL (for HepG2 cells), and 23.7 mug/mL (for Eca-109 cells). Further study of K562 cells involving fluorescence and transmission electron microscopy revealed characteristic apoptotic features, such as cell shrinkage, membrane blebbing, loss of microvilli, and nuclear condensation. Agarose electrophoresis of genomic DNA showed a typical fragmentation pattern for apoptotic cells. A kinetic cell-cycle analysis demonstrated that the cell cycle was arrested in the G1 phase. All these results suggest that the anti-proliferation effect of jolkinolide B on K562 cells is achieved by arresting the cell cycle in the G1 phase and subsequently inducing apoptosis.
机译：Jolkinolide B是一种从大戟大戟根中分离出来的生物活性二萜，具有多种生物学和药理特性。在这项研究中，在人类慢性粒细胞白血病（K562）和其他2种细胞系（人类食道癌Eca-109和人类肝癌HepG2）中测试了高度纯化的jolkinolide B的细胞毒性。结果表明，当用jolkinolide B处理24 h时，所有3种细胞系的增殖均显着降低。细胞毒性的IC50值是12.1杯/毫升（对于K562细胞），> 50.0杯/毫升（对于HepG2细胞）和23.7杯/毫升（对于Eca-109细胞）。对涉及荧光和透射电子显微镜的K562细胞的进一步研究揭示了特征性的凋亡特征，例如细胞收缩，膜起泡，微绒毛丧失和核凝聚。基因组DNA的琼脂糖电泳显示凋亡细胞具有典型的片段化模式。动力学细胞周期分析表明，细胞周期被阻滞在G1期。所有这些结果表明，通过将细胞周期停滞在G1期并随后诱导凋亡，可以实现jolkinolide B对K562细胞的抗增殖作用。

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