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首页> 外文期刊>Canadian Journal of Physiology and Pharmacology >Expression of breast cancer resistance protein (BCRP/ABCG2) in human placenta throughout gestation and at term before and after labor.
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Expression of breast cancer resistance protein (BCRP/ABCG2) in human placenta throughout gestation and at term before and after labor.

机译：整个妊娠期间以及分娩前后的胎盘中，乳腺癌抗性蛋白（BCRP / ABCG2）的表达。

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摘要

Breast cancer resistance protein, BCRP, is a multidrug resistance protein that is highly expressed in the human placenta. In cancer tissues, this protein actively extrudes a wide variety of chemically and structurally unrelated chemotherapeutic drugs and other compounds. Studies in mice have shown that in the absence of BCRP activity in the placenta, there is a 2-fold increase in the uptake in BCRP substrates into fetus. This suggests that in the placenta, BCRP extrudes compounds that would otherwise cross the syncytiotrophoblast cells into fetal circulation. The purpose of this study was to examine the expression and localization of BCRP in the human placenta throughout gestation. Tissues from 6-13, 16-19, 24-29, 32-35, and 38-41 weeks of gestation were used. Real time RT-PCR analysis demonstrated that the mRNA levels of BCRP in the placenta do not change significantly as gestation progressed. However, Western blot analysis revealed that the protein levels increased towards the end of gestation. We demonstrated that BCRP is localized to the syncytiotrophoblast of the placenta and in some fetal blood vessels within the placenta. Tissues from the early stages of pregnancy (6-13 weeks) showed fewer BCRP positive blood vessels than term tissues (38-41 weeks).
机译：乳腺癌抗性蛋白BCRP是一种在人类胎盘中高度表达的多药抗性蛋白。在癌症组织中，这种蛋白质可主动挤出多种化学和结构无关的化学治疗药物和其他化合物。小鼠研究表明，在胎盘中缺乏BCRP活性的情况下，胎儿对BCRP底物的摄取增加了2倍。这表明，在胎盘中，BCRP会挤出化合物，否则这些化合物会穿过合体滋养层细胞进入胎儿循环。这项研究的目的是检查整个妊娠过程中人胎盘中BCRP的表达和定位。使用妊娠6-13、16-19、24-29、32-35和38-41周的组织。实时RT-PCR分析表明，胎盘中BCRP的mRNA水平不会随着妊娠的进行而发生明显变化。但是，蛋白质印迹分析表明，蛋白质水平在妊娠快要结束时增加。我们证明了BCRP定位于胎盘的合体滋养细胞和胎盘内的某些胎儿血管中。妊娠早期（6-13周）的组织显示的BCRP阳性血管少于足月组织（38-41周）。

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