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首页> 外文期刊>Canadian Journal of Physiology and Pharmacology >Current concepts in nuclear pore electrophysiology.
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Current concepts in nuclear pore electrophysiology.

机译：核孔电生理学的当前概念。

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Over 4 decades ago, microelectrode studies of in situ nuclei showed that, under certain conditions, the nuclear envelope (NE) behaves as a barrier opposing the nucleocytoplasmic flow of physiological ions. As the nuclear pore complexes (NPCs) of the NE are the only pathways for direct nucleocytoplasmic flow, those experiments implied that the NPCs are capable of restricting ion flow. These early studies validated electrophysiology as a useful approach to quantify some of the mechanisms by which NPCs mediate gene activity and expression. Since electron microscopy (EM) and other non-electrophysiological investigations, showed that the NPC lumen is a nanochannel, the opinion prevailed that the NPC could not oppose the flow of ions and, therefore, that electrophysiological observations resulted from technical artifacts. Consequently, the initial enthusiasm with nuclear electrophysiology faded out in less than a decade. In 1990, nuclear electrophysiology was revisited with patch-clamp, the most powerful electrophysiological technique to date. Patch-clamp has consistently demonstrated that the NE has intrinsic ion channel activity. Direct demonstrations of the NPC on-off ion channel gating behavior were published for artificial conditions in 1995 and for intact living nuclei in 2002. This on-off switching/gating behavior can be interpreted in terms of a metastable energy barrier. In the hope of advancing nuclear electrophysiology, and to complement the other papers contained in this special issue of the journal, here I review some of the main technical, experimental, and theoretical issues of the field, with special focus on NPCs.
机译：超过四十年前，对原位核的微电极研究表明，在某些条件下，核膜（NE）充当了阻碍生理离子核质流的屏障。由于NE的核孔复合物（NPC）是直接核质流的唯一途径，因此这些实验表明NPC能够限制离子流。这些早期研究证实电生理学是量化NPC介导基因活性和表达的某些机制的有用方法。由于电子显微镜（EM）和其他非电生理研究表明NPC内腔是一个纳米通道，因此，人们普遍认为NPC不能抵抗离子流，因此，电生理观察是由技术伪像引起的。因此，在不到十年的时间里，人们对核电生理学的最初热情就消失了。 1990年，膜片钳重新审视了核电生理，这是迄今为止最强大的电生理技术。膜片钳一直证明NE具有固有的离子通道活性。 NPC开-关离子通道门控行为的直接论证于1995年在人工条件下发表，而在2002年则用于完整的活核。这种开/关开关/门控行为可以用亚稳能垒来解释。为了促进核电生理学发展，并补充本期杂志的特刊，在这里，我回顾了该领域的一些主要技术，实验和理论问题，特别关注NPC。

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