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首页> 外文期刊>Canadian Journal of Physiology and Pharmacology >Nonsurgically induced disuse muscle atrophy and neuromuscular dysfunction upregulates alpha7 acetylcholine receptors
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Nonsurgically induced disuse muscle atrophy and neuromuscular dysfunction upregulates alpha7 acetylcholine receptors

机译：非手术引起的废用性肌肉萎缩和神经肌肉功能障碍上调α7乙酰胆碱受体

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Previous models of muscle disuse have invariably used surgical methods that require the repetitive application of plaster casts. A method of disuse atrophy that does not require such repetitive applications is described herein. Modified plastic pipette tubing was applied to a single hindlimb (mouse), from thigh to foot, resulting in immobilization of the knee in the extension position, and the ankle in the plantar flexion position. This method resulted in the loss of soleus muscle to 11%, 22%, 39%, and 45% of its original mass at 3, 7, 14, and 21 days, respectively, in association with a significant decrease of tibialis twitch (25%) and tetanic tensions (26%) at 21 days, compared with the contralateral side and (or) sham-immobilized controls. Immuno-histochemical analysis of the soleus using fluorescent α-bungarotoxin revealed a significant increase in the number of synapses per unit area (818 + 31 compared with 433 + 16/mm2) and an increase in muscle fibers per unit area (117 compared with 83/mm2), most likely related to the atrophy of muscle fibers bringing synapses closer. A 3-fold increase in alpha7 acetylcholine receptor (α7AChR) protein expression, along with increased expression of α1AChR subunit in the immobilized side compared with the contralateral side was observed. The physiology and pharmacology of the novel finding of upregulation of α7AChRs with disuse requires further study.
机译：以前的肌肉废用模型总是使用外科手术方法，这些方法需要重复使用石膏模型。本文描述了不需要这种重复应用的废用萎缩方法。从大腿到脚，将改良的塑料移液管应用到单个后肢（鼠标）上，从而将膝盖固定在伸展位置，并将脚踝固定在plant屈位置。这种方法导致比目鱼肌在3、7、14和21天分别损失至其原始质量的11％，22％，39％和45％，同时胫骨抽搐明显减少（25 ％）和21天的破伤风张力（26％），与对侧和/或假手术固定对照相比。比目鱼的免疫组织化学分析使用荧光α-邦加罗毒素显示，每单位面积的突触数量显着增加（818 + 31，而433 + 16 / mm2），每单位面积的肌纤维增加（117，83 / mm2），最有可能与肌肉纤维萎缩导致突触更近有关。与对侧相比，固定侧的α7乙酰胆碱受体（α7AChR）蛋白表达增加了3倍，而α1AChR亚基的表达也增加了。废弃使用中α7AChRs上调的新发现的生理学和药理学需要进一步研究。

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