...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Canadian Journal of Physiology and Pharmacology >Diosgenin does not express estrogenic activity: A uterotrophic assay
【24h】

Diosgenin does not express estrogenic activity: A uterotrophic assay

机译：薯gen皂素不表达雌激素活性：子宫营养测定

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

This study assessed the effects of diosgenin on estrogenic activity using a uterotrophic assay. Immature female rats received diosgenin orally at doses of 200, 100, or 20 mg/kg body mass; and 17α ethynylestradiol at doses of 1 or 0.3 μg/kg, daily, for 3 consecutive days from day 19 to day 21. Controls were distributed among 2 groups: an intact control group and a vehicle control group. Animals were sacrificed 24 h after the last application of diosgenin, estradiol, or vehicle (22nd day of life). Uterine wet weight, stereological and histomorphometrical changes, immunohistochemical expression of estrogen receptor alpha (ERα), progesterone receptor (PR), and the expression of lactoferrin (LF) were examined. Diosgenin did not affect the uterine wet weight, epithelium height, volume densities of endometrium, endometrial epithelia, number of endometrial glands, or histological appearance of vaginal epithelia. ERα, PR, and LF immunostaining intensity were not altered in the animals that received diosgenin. High-potency reference ER agonist 17α-ethynylestradiol induced a significant increase in all of the measured parameters, and as expected, decreased ERα immunostaining intensity. Based on these data, it can be concluded that diosgenin, at doses of 20-200 mg/kg, did not act as an estrogen agonist in the immature rat uterotrophic assay.
机译：这项研究使用子宫营养法评估薯os皂甙元对雌激素活性的影响。未成年雌性大鼠口服薯di皂甙元剂量为200、100或20 mg / kg体重;从第19天到第21天，连续3天每天服用1或0.3μg/ kg的17α乙炔雌二醇和对照组。对照组分为两组：完整对照组和媒介对照组。最后一次使用薯os皂苷元，雌二醇或媒介物（生命第22天）后24小时处死动物。检查子宫湿重，体貌和组织形态学变化，雌激素受体α（ERα），孕激素受体（PR）的免疫组织化学表达以及乳铁蛋白（LF）的表达。薯os皂素不会影响子宫的湿重，上皮高度，子宫内膜的体积密度，子宫内膜上皮细胞，子宫内膜腺的数量或阴道上皮的组织学外观。接受薯os皂苷元的动物的ERα，PR和LF免疫染色强度没有改变。高能参考ER激动剂17α-乙炔基雌二醇诱导所有测量参数的显着增加，并且正如预期的那样，降低了ERα免疫染色强度。根据这些数据，可以得出结论，薯os皂甙元在20-200 mg / kg的剂量下，在未成熟的大鼠子宫营养试验中并未起雌激素激动剂的作用。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号