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首页> 外文期刊>Fish Physiology and Biochemistry >Effects of a Dissostichus mawsoni-CaM recombinant proteins feed additive on the juvenile orange-spotted grouper (Epinephelus coioides) under the acute low temperature challenge
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Effects of a Dissostichus mawsoni-CaM recombinant proteins feed additive on the juvenile orange-spotted grouper (Epinephelus coioides) under the acute low temperature challenge

机译：Dissostichus mawsoni-CaM重组蛋白饲料添加剂对急性低温攻击下的橙斑斑斑石斑鱼（Epinephelus coioides）的影响

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摘要

The effects of Dissostichus mawsoni-Calmodulin (Dm-CaM) on growth performance, enzyme activities, respiratory burst, MDA level and immune-related gene expressions of the orange-spotted grouper (Epinephelus coioides) exposed to the acute low temperature stress were evaluated. The commercial diet supplemented with Dm-CaM protein was fed to the groupers for 6 weeks. No significant difference was observed in the specific growth rates, weight gains and survivals. After the feeding trial, the groupers were exposed to acute low temperature challenge. The groupers fed with Dm-CaM additive diet showed a significant decrease in the respiratory burst activity, while the blood cell number increased significantly at 25 degrees C by comparing with the control and additive control group. The enzymatic activity of SOD, ACP and ALP increased significantly in Dm-CaM additive group, while MDA level maintained stable with the lowest value. qRT-PCR analysis indicated that the up-regulated transcript expressions of CaM, C3, SOD2, LysC and HSPA4 were observed in Dm-CaM additive group. These results indicated that Dm-CaM additive diet may regulate the grouper immune response to the acute low temperature challenge.
机译：评估了Dissostichus mawsoni-钙调蛋白（Dm-CaM）对暴露于急性低温胁迫下的橙色斑斑石斑鱼（Epinephelus coioides）的生长性能，酶活性，呼吸爆发，MDA水平和免疫相关基因表达的影响。将补充有Dm-CaM蛋白的商业饮食喂给石斑鱼6周。在特定的生长速率，体重增加和存活率上没有观察到显着差异。喂食试验后，石斑鱼暴露于急性低温刺激下。与对照组和添加剂对照组相比，喂食Dm-CaM添加剂饮食的石斑鱼的呼吸爆发活性显着下降，而在25摄氏度时血细胞数量显着增加。 Dm-CaM添加剂组中SOD，ACP和ALP的酶活性显着增加，而MDA水平保持稳定，最低。 qRT-PCR分析表明，Dm-CaM添加剂组中CaM，C3，SOD2，LysC和HSPA4的转录本表达上调。这些结果表明，Dm-CaM添加剂饮食可调节石斑鱼对急性低温挑战的免疫反应。

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