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首页> 外文期刊>Biochemical Systematics and Ecology >A combined approach using RAPD, ISSR and volatile analysis for the characterization of Thymus caespititius from Flores, Corvo and Graciosa islands (Azores, Portugal)
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A combined approach using RAPD, ISSR and volatile analysis for the characterization of Thymus caespititius from Flores, Corvo and Graciosa islands (Azores, Portugal)

机译：使用RAPD，ISSR和挥发性分析的组合方法表征弗洛雷斯，科尔沃和格拉乔萨群岛（葡萄牙亚速尔群岛）的百里香

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摘要

RAPDs and ISSRs were used as molecular markers and combined with the chemical analysis of the volatiles, aiming at characterizing Thymus caespititius from the islands of Corvo, Flores and Graciosa (Azores). Thirty-one individuals were analyzed using 17 RAPD and 11 ISSRs primers, which generated respectively 199 and 127 polymorphic scorable bands. Volatiles were isolated by distillation-extraction and analyzed by GC and GC-MS. Molecular data obtained from both markers discriminated assessments from Corvo and Flores which clustered separately from plants collected in Graciosa. Volatile data grouped plants in a similar way, giving rise to two groups, depending on their oil type; plants collected on Corvo and Flores yielded carvacrol-rich volatiles while those from Graciosa yielded both carvacrol and alpha -terpineol-rich oils.
机译：RAPD和ISSR被用作分子标记，并与挥发物的化学分析相结合，旨在表征来自Corvo，Flores和Graciosa（亚速尔群岛）岛屿的百里香。使用17个RAPD和11个ISSRs引物分析了31个个体，分别产生了199和127个多态可评分带。通过蒸馏萃取分离挥发物，并通过GC和GC-MS分析。从这两种标记物获得的分子数据区分了来自Corvo和Flores的评估，后者分别与Graciosa中收集的植物聚在一起。挥发性数据以相似的方式对植物进行分组，根据其油的类型将其分为两组。在Corvo和Flores上收集的植物产生了富含香芹酚的挥发物，而从Graciosa收集的植物则产生了香芹酚和富含α-萜品醇的油。

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