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首页> 外文期刊>Fisheries and Aquatic Sciences >A Myostain-like Gene Expressed Highly in the Muscle Tissue of Chinese mitten crab, Eriocheir sinensis
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A Myostain-like Gene Expressed Highly in the Muscle Tissue of Chinese mitten crab, Eriocheir sinensis

机译：在中华绒螯蟹中华绒螯蟹肌肉组织中高表达的类肌变蛋白基因

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摘要

A complete cDNA, which encodes for a myostatin-like protein (Es-MSTN), was isolated from the Chinese mitten crab, Eriocheir sinensis. Es-MSTN was composed of 2,397 nucleotides and the open reading frame (ORF) specified a protein containing 468 amino acids. Es-MSTN exhibited 32% amino acid sequence identity and 52% similarity to human myostatin. Multiple sequence alignment analysis indicated that Es-MSTN possessed the conserved proteolytic cleavage site (RXXR) for maturation of the protein and nine cysteine residues for disulfide bridges. Besides the conserved structural features, Es-MSTN also exhibits its unique characters; a longer N-terminal domain which is involved in protein folding and latent form of myostatin and absence of the cleavage site for BMP-l/tolloid family of metalloproteihase to activate mature myostatin. Phylogenetic analysis suggests that Es-MSTN showed the closely related to both vertebrate myostatin and GDF11. Es-MSTN is expressed highly in the claw muscle, leg muscle, thoracicmuscle and heart, and moderately in the hindgut suggesting that Es-MSTN may play important roles in the muscle tissues. As homolog of mammalian myostatin and GDF11, Es-MSTN may be involved in development of muscular tissue and further study will help toproduce high-quality seafood.
机译：从中华绒螯蟹中华绒螯蟹中分离出一个完整的cDNA，该蛋白编码一种肌生长抑制素样蛋白（Es-MSTN）。 Es-MSTN由2,397个核苷酸组成，开放阅读框（ORF）指定一种包含468个氨基酸的蛋白质。 Es-MSTN与人肌肉生长抑制素的氨基酸序列同一性为32％，相似性为52％。多重序列比对分析表明，Es-MSTN具有保守的蛋白水解切割位点（RXXR）来使蛋白质成熟，并具有九个半胱氨酸残基用于二硫键。 Es-MSTN除了具有保守的结构特征外，还表现出其独特的特点。一个较长的N-末端结构域，其参与肌肉生长抑制素的蛋白折叠和潜在形式，并且不存在金属蛋白酶的BMP-1 /类脂家族的切割位点以激活成熟的肌肉生长抑制素。系统发育分析表明，Es-MSTN与脊椎动物的肌生长抑制素和GDF11密切相关。 Es-MSTN在爪肌，腿部肌肉，胸肌和心脏中高表达，在后肠中度表达，表明Es-MSTN在肌肉组织中可能起重要作用。作为哺乳动物肌生长抑制素和GDF11的同源物，Es-MSTN可能参与肌肉组织的发育，进一步的研究将有助于生产高质量的海鲜。

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