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首页> 外文期刊>Fisheries and Aquatic Sciences >Detection of Laminariaceae Species Based on PCR by Family-specific ITS Primers
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Detection of Laminariaceae Species Based on PCR by Family-specific ITS Primers

机译：家庭特异性ITS引物基于PCR的海带科物种检测

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摘要

To analyze nucleotide sequence encoding internal transcribed spacer (ITS) regions specific to the Laminariaceae family, genomic DNA was isolated from six brown algae species distributed along the east coast of Korea. These included three species fromthe Laminariaceae family (Agarum clathratum Dumortier, Costaria costata [C. Agardh] Saunders, and Saccharina japonica Are-schoug) and two species from the Alariaceae family (Undaria pinnatifida [Harvey] Suringer and Ecklonia cava Kjellman), both in the order Laminariales, and one species from the family Sargassaceae in the order Fucales (Sargassum serratifolium). Based on a sequence analysis of ITS-1 and ITS-2 for A. clathratum, C. costata, and E. cava, oligonucleotides were designed from the regions that showed sequence conservation in Laminariaceae. Following polymerase chain reaction using three sets of primers, amplification of ITS-1 and ITS-2 was detected in reactions using genomic DNA isolated from the species belonging to Laminariaceae, but notfrom the species belonging to the other families. The results indicate that this method can be used for the detection and identification of Laminariaceae species.
机译：为了分析编码对海带科特有的内部转录间隔区（ITS）区域的核苷酸序列，从分布在韩国东海岸的六个褐藻物种中分离了基因组DNA。其中包括来自海带科（Agarum clathratum Dumortier，Cosaria costata [C. Agardh] Saunders和Saccharina japonica Are-schoug）的三种，以及来自Alariaceae族的两种（Undaria pinnatifida [Harvey] Suringer和Ecklonia cava Kjellman）。属于海带纲，以及一种来自富足纲（Sargassum serratifolium）的Sargassaceae科的物种。基于对clathratum，C.costata和E.cava的ITS-1和ITS-2的序列分析，从显示了海带科序列保守性的区域设计了寡核苷酸。在使用三组引物进行的聚合酶链式反应之后，在使用分离自海带科物种而不是其他科目的物种的基因组DNA的反应中检测到ITS-1和ITS-2的扩增。结果表明该方法可用于海带科的检测和鉴定。

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