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首页> 外文期刊>Fisheries and Aquatic Sciences >Growth Performance of Transgenic Mud Loach Misgurnus mizolepis Carrying a GH Transgene Driven by Mud Loach C-Type Lectin Regulator
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Growth Performance of Transgenic Mud Loach Misgurnus mizolepis Carrying a GH Transgene Driven by Mud Loach C-Type Lectin Regulator

机译：携带泥LoC型凝集素调节剂驱动GH转基因的转基因泥LoMisgurnus mizolepis的生长性能

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摘要

Growth hormone (GH) transgenesis in fish has the potential to improve aquaculture efficiency and capacity. However, many fast-growing transgenic fish have experienced side effects caused by excess GH expression. To overcome this unwanted issue associated with several GH transgenic mud loach Misgurnus mizolepis lines carrying GH construct driven by a strong P-actin regulator (pml beta-actGH), we performed an alternative version of GH autotransgenesis using a weaker but more stable regulator, the mud loach lectin promoter. GH transgenesis with a pmlectGH construct consisting of the mud loach GH gene driven by the 2.3-kb lectin promoter exhibited significant growth stimulation. However, the extent of the growth acceleration in pmlectGH transgenics (sixtimes maximum when assessed 2 months post hatching) was much less than that in transgenic individuals carrying the pmlp-actGH construct. Additionally, the extraordinary gigantism that-was common in pmip-actGH-transgenic mud loaches was diminished in transgenic loaches harboring the pmlectGH construct. Transgenic founders (pmlectGH) successfully transmitted their transgene into the next generation with up to 41% frequency. Growth stimulation also persisted in the transgenic F1 strains, with a seven-foldincrease in maximum body weight at 6 months of age.
机译：鱼类中生长激素（GH）的转基因具有提高水产养殖效率和能力的潜力。但是，许多快速生长的转基因鱼都因过量的GH表达而产生了副作用。为了克服与几个由强P-肌动蛋白调节剂（pml beta-actGH）驱动的GH构造的GH转基因泥lo Misgurnus mizolepis品系相关的不想要的问题，我们使用了一个较弱但更稳定的调节剂进行了GH自转基因的替代版本，泥lo凝集素启动子。由由2.3-kb凝集素启动子驱动的泥loGH基因组成的pmlectGH构建体进行的GH转基因表现出显着的生长刺激。但是，pmlectGH转基因的生长加速程度（孵化后2个月评估为最大六倍）远小于携带pmlp-actGH构建体的转基因个体。另外，在具有pmlectGH构建体的转基因泥ache中，在pmip-actGH-转基因泥lo中非常常见的巨大现象被消除。转基因创始人（pmlectGH）成功地将其转基因以高达41％的频率传递给了下一代。转基因F1菌株也持续生长，在6个月大时最大体重增加了7倍。

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