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首页> 外文期刊>Fish Physiology and Biochemistry >Acute effects of cadmium on liver phase I and phase II enzymes and metallothionein accumulation on sea bream Sparus aurata
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Acute effects of cadmium on liver phase I and phase II enzymes and metallothionein accumulation on sea bream Sparus aurata

机译：镉对鲷鱼Sparus aurata肝脏I，II期酶和金属硫蛋白积累的急性影响

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This research was designed to study Sparus aurata (sea bream) biotransformation and detoxification responses to acute exposure to cadmium (Cd). Sexually immature gilthead sea bream were treated by intraperitoneal injection of Cd chloride (200 og kgp#) for 6, 12, 24 and 48 h. Cd accumulation was quantified in sea bream liver by graphite furnace atomic absorption spectroscopy after the various exposure periods. The following biological responses were measured: (1) ethoxyresorufin-O-deethylase (EROD) activity as phase I biotransformation parameter, (2) liver glutathione-S-transferase (GST) activity as a phase II conjugation enzyme and metallothionein (MT) content as specific response to Cd contamination. Cd bioaccumulation in the liver resulted in an increasing uptake up to 10.3 og gp# wet weight after 48 h of exposure. EROD showed a significant activation only after 6 h exposure and a return to control levels after 12 h. GST revealed significant activation starting from 12 h exposure. MT accumulation in liver showed the same behavior as GST activation.
机译：这项研究旨在研究鲷鱼（Sparus aurata）的生物转化和对急性镉（Cd）的排毒反应。通过腹膜内注射氯化镉（200 ug kgp＃）治疗性不成熟的金头鲷6、12、24和48小时。在不同的暴露时间后，通过石墨炉原子吸收光谱法对鲷鱼肝中的镉积累进行定量。测量了以下生物学反应：（1）乙氧基异佛手菌素-O-脱乙基酶（EROD）活性作为I期生物转化参数，（2）肝谷胱甘肽S-转移酶（GST）活性作为II期结合酶和金属硫蛋白（MT）含量作为对镉污染的具体反应。暴露48小时后，肝脏中Cd的生物蓄积导致吸收增加，湿重达10.3 og gp＃。 EROD仅在暴露6 h后才显示出明显的激活，并在12 h后恢复到对照水平。 GST从暴露12 h开始显示出明显的激活。肝脏中MT的积累表现出与GST激活相同的行为。

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