...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Canadian Journal of Physiology and Pharmacology >Decreased RhoA expression in myocardium of diabetic rats.
【24h】

Decreased RhoA expression in myocardium of diabetic rats.

机译：糖尿病大鼠心肌中RhoA表达的降低。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Diabetic cardiomyopathy is 1 of the major causes of death in diabetic patients, but the pathogenesis is unclear. There is evidence that RhoA, a small GTPase, might be involved in cardiac function. This study, therefore, analyzed RhoA expression and activation in hearts of diabetic rats. Male Sprague–Dawley rats were divided into control and diabetic groups of 18 each. Diabetes was induced by intravenous injection of streptozotocin (55 mg/kg). Rats were studied 3 weeks after induction of diabetes. Heart rate, which was measured 24 h/day, decreased by 93 +/- 7 beats/min in diabetic rats. There was a 62% decrease (p 0.01) in RhoA mRNA expression in heart tissues (left ventricle) of diabetic rats (38.5 +/- 6.7 x 106 molecules/microg total RNA) compared with controls (101 +/- 10.3 x 106 molecules/microg total RNA). Western blot showed a 33% decrease in total RhoA protein expression in heart tissues of diabetic rats compared with controls (p 0.05). A reduced RhoA translocation in heart tissues of diabetic rats was determined by a 64% decrease in membrane-bound RhoA (p 0.01 vs. control group), indicating that the activation of RhoA is markedly reduced in diabetic myocardium. Our data suggest that down-regulated RhoA may be involved in cardiomyopathy in diabetic rats.
机译：糖尿病性心肌病是糖尿病患者死亡的主要原因之一，但发病机理尚不清楚。有证据表明，小的GTP酶RhoA可能与心脏功能有关。因此，该研究分析了糖尿病大鼠心脏中RhoA的表达和激活。将雄性Sprague–Dawley大鼠分为对照组和糖尿病组，每组18只。静脉注射链脲佐菌素（55 mg / kg）可诱发糖尿病。在诱导糖尿病后3周对大鼠进行研究。在糖尿病大鼠中，每天24小时测量的心率降低了93 +/- 7次/分钟。与对照组（101 +/- 10.3 x 106）相比，糖尿病大鼠心脏组织（左心室）中的RhoA mRNA表达降低了62％（p <0.01）（38.5 +/- 6.7 x 106分子/微克总RNA）。分子/微克总RNA）。 Western印迹显示，与对照组相比，糖尿病大鼠心脏组织中的总RhoA蛋白表达降低了33％（p <0.05）。糖尿病大鼠心脏组织中RhoA易位的降低是通过膜结合RhoA降低64％来确定的（与对照组相比，p <0.01），表明糖尿病心肌中RhoA的激活显着降低。我们的数据表明，RhoA的下调可能与糖尿病大鼠的心肌病有关。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号