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首页> 外文期刊>Canadian Journal of Physiology and Pharmacology >The effect of portacaval anastomosis on the expression of glutamine synthetase and ornithine aminotransferase in perivenous hepatocytes
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The effect of portacaval anastomosis on the expression of glutamine synthetase and ornithine aminotransferase in perivenous hepatocytes

机译：门腔静脉吻合术对静脉肝细胞谷氨酰胺合成酶和鸟氨酸氨基转移酶表达的影响

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摘要

There is functional zonation of metabolism across the liver acinus, with glutamine synthetase restricted to a narrow band of cells around the terminal hepatic venules. Portacaval anastomosis, where there is a major rerouting of portal blood flow from the portal vein directly to the vena cava bypassing the liver, has been reported to result in a marked decrease in the activity of glutamine synthetase. It is not known whether this represents a loss of perivenous hepatocytes or whether there is a specific loss of glutamine synthetase. To answer this question, we have determined the activity of glutamine synthetase and another enzyme from the perivenous compartment, ornithine aminotransferase, as well as the immunochemical localization of both glutamine synthetase and ornithine aminotransferase in rats with a portacaval shunt. The portacaval shunt caused a marked decrease in glutamine synthetase activity and an increase in ornithine aminotransferase activity. Immunohistochemical analysis showed that the glutamine synthetase and ornithine aminotransferase proteins maintained their location in the perivenous cells. These results indicate that there is no generalized loss of perivenous hepatocytes, but rather, there is a significant alteration in the expression of these proteins and hence metabolism in this cell population.
机译：整个肝腺泡有新陈代谢的功能区带，谷氨酰胺合成酶被限制在末梢肝小静脉周围的狭窄细胞带中。据报道门腔吻合术中，门静脉血流主要从门静脉直接转移到绕过肝脏的腔静脉，导致谷氨酰胺合成酶活性显着降低。尚不知道这是否代表静脉肝细胞的丢失或是否存在谷氨酰胺合成酶的特定丢失。为了回答这个问题，我们确定了具有门腔分流的大鼠中谷氨酰胺合成酶和另一种来自静脉腔的酶的活性，鸟氨酸氨基转移酶，以及谷氨酰胺合成酶和鸟氨酸氨基转移酶的免疫化学定位。门腔分流导致谷氨酰胺合成酶活性明显降低，鸟氨酸氨基转移酶活性升高。免疫组织化学分析表明谷氨酰胺合成酶和鸟氨酸氨基转移酶蛋白在静脉细胞中保持其位置。这些结果表明，没有静脉肝细胞的普遍丢失，但是，这些蛋白质的表达发生了显着改变，因此该细胞群中的代谢也发生了显着改变。

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