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首页> 外文期刊>Biochemical Systematics and Ecology >Molecular characterization and mitochondrial sepuence variation in two sympatric species of Proechimys (Rodentia:Echimyidae) in french guiana
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Molecular characterization and mitochondrial sepuence variation in two sympatric species of Proechimys (Rodentia:Echimyidae) in french guiana

机译：法属圭亚那两种原虫的同伴物种的分子表征和线粒体变异

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摘要

Variations in mitochondrial DNA characters were used to characterize two morphologically similar and sympatric species of Neotropical terrestrial rodents of the genus Proechimys (Mammalia: Echimyidae). We sepuenced both cytochrome b (1140pb) and part of the control region (445pb) from four individuals of P. cuvieri and five of p. cayennensis from French Guiana, which allowed us to depict intra- and inter-specific patterns of variation. The phylogenetic relationships between the nine sequences evidence the monophyly of each species, and illustrate that more polymorphism might exist within P. cuvieri than within P. cayennensis. By developing specific primers to amplify a fragment of the cytochrome b gene, we were able to identify 50 individuals of Proechimys spp. caught in two localities of French Guiana. In both sites of primary rainforests. we showed that the twoseceies ilve in syntopy, and this observation emphasizes the need to document ecological differences which should exist in order to diminish inter-specific competition.
机译：线粒体DNA特征的变化被用来表征Proechimys属（哺乳动物：Echimyidae）的两种形态相似和同生的新热带陆地啮齿动物。我们分离了来自四个P. cuvieri和五个p。cuvieri的细胞色素b（1140pb）和部分对照区域（445pb）。来自法属圭亚那的cayennensis，使我们能够描绘种内和种间变异模式。九个序列之间的系统发育关系证明了每个物种的单性，并表明在Cu。cuvieri中可能比在C. cayennensis中存在更多的多态性。通过开发特异性引物来扩增细胞色素b基因的片段，我们能够鉴定出50个Proechimys spp个体。在法属圭亚那的两个地方被捕。在两个主要的热带雨林地区。我们证明了这两个部分是同义的，并且这一观察结果强调了需要记录生态差异的必要性，以减少种间竞争。

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