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Potato in Vitro Culture Techniques and Biotechnology

机译：马铃薯体外培养技术与生物技术

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摘要

This review was prepared as a survey of key articles presenting development, achievements and interconnection of various lines of potato biotechnology research united through the common use of in vitro culture techniques. Starting with the early research on the induction and differentiation of callus tissues, review sequentially and chronologically presents the advance of various in vitro culture techniques and their practical applications in clonal propagation, germplasm storage, production of healthy virus-free plants and breeding. Main topics (sections) presented include: establishment and maintenance of callus, root and shoot cultures; variability in callus tissue and protoplast cultures; shoot differentiation and regeneration from various explant types and cultures, somatic embryogenesis and androgenesis; shoot and meristem tip cultures and their numerous applications; regulation of in vitro tuberization, microtubers dormancy and sprouting and systems for production of micro and minitubers. Finally, review outlines the gradual technique merging and shifting of research interest from cellular to molecular biology. This approach enables us to comprehend developmental processes in cells and tissues not only through the interaction of plant growth regulators and environmental factors but also through sequential activation and expression of regulatory genes.
机译：这篇综述是作为对关键文章的调查而编写的，这些论文介绍了通过共同使用体外培养技术而联合起来的马铃薯生物技术研究的各个领域的发展，成就和相互联系。从对愈伤组织诱导和分化的早期研究开始，按顺序和时间顺序回顾了各种体外培养技术的进展及其在克隆繁殖，种质储存，健康无病毒植物的生产和育种中的实际应用。提出的主要主题（部分）包括：愈伤组织，根和芽培养物的建立和维持;愈伤组织和原生质体培养物中的变异性;从各种外植体类型和培养，体细胞胚发生和雄激素发生中分化和再生芽;射击和分生组织尖端培养及其广泛应用;调节体外块茎，微块茎的休眠和发芽以及生产微块茎和小块茎的系统。最后，综述概述了渐进技术的融合以及研究兴趣从细胞生物学到分子生物学的转移。这种方法使我们不仅可以通过植物生长调节剂和环境因素的相互作用，而且可以通过顺序激活和表达调节基因来理解细胞和组织中的发育过程。

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