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首页> 外文期刊>Biochemical Systematics and Ecology >Biochemical characterization of extracellular polymeric substances extracted from an intertidal mudflat using a cation exchange resin
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Biochemical characterization of extracellular polymeric substances extracted from an intertidal mudflat using a cation exchange resin

机译：使用阳离子交换树脂从潮间带滩涂提取的细胞外聚合物的生化特性

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摘要

The biochemical characterization of Extracellular Polymeric Substances (EPS) excreted in a European intertidal mudflat (Marennes-Oleron Bay) was performed. Experiments were carried out for the first time in situ, by using an improved extraction recently developed. This innovative procedure, using a cation exchange resin (Dowex), allows separating precisely different fractions of EPS, especially pure bound EPS. Moreover, it avoids the contamination of EPS fractions by residual and intracellular polymers, enabling to properly estimate polymeric contents in each fraction. The results were partly similar to conventional results described in the literature and the amount of colloidal carbohydrates (146 mu g/g of dry sediment) extracted by the Dowex method fitted well with different EPS estimation in European mudflats. Colloidal carbohydrates were essentially composed of glucose (>50%), a carbon source rapidly consumed by the various communities in the sediment. Pure bound carbohydrates were composed of specific carbohydrates (28% rhamnose, 22% xylose). Residual fractions, considered as containing some refractory bound EPS and mostly other internal polymeric substances, presented a more varied composition rich in carbohydrates: galacturonic acid (20%), mannose (19.5%), glucose (19%), arabinose (15%), xylose (8%), galactose (7%).
机译：进行了欧洲潮间带滩涂（Marennes-Oleron湾）中排出的细胞外聚合物（EPS）的生化表征。通过使用最近开发的改进提取方法，首次在原位进行了实验。这种使用阳离子交换树脂（Dowex）的创新方法可以分离出EPS的不同部分，尤其是纯结合的EPS。此外，它避免了残留和细胞内聚合物对EPS组分的污染，从而能够正确估算每个组分中的聚合物含量。结果与文献中描述的常规结果部分相似，并且通过Dowex方法提取的胶体碳水化合物的量（146μg / g干沉积物）与欧洲泥滩的EPS估算值非常吻合。胶体碳水化合物主要由葡萄糖（> 50％）组成，葡萄糖是沉积物中各个群落迅速消耗的碳源。纯结合的碳水化合物由特定的碳水化合物组成（28％鼠李糖，22％木糖）。残留级分，被认为包含一些难熔结合的EPS和大多数其他内部聚合物质，其组成成分更加多样化，富含碳水化合物：半乳糖醛酸（20％），甘露糖（19.5％），葡萄糖（19％），阿拉伯糖（15％） ，木糖（8％），半乳糖（7％）。

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