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首页> 外文期刊>Biochemical Systematics and Ecology >Estimation of genetic variation in Cynodon dactylon accessions using the ISSR technique
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Estimation of genetic variation in Cynodon dactylon accessions using the ISSR technique

机译：利用ISSR技术估算狗牙根克隆的遗传变异。

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Genetic variation in 55 accessions of Cynodon dactylon was estimated using inter-simple sequence repeat (ISSR) markers. The plant materials used in this study originated from 17 countries. A total of 236 ISSR fragments were generated with 14 primers. Fragment sizes ranged from 200 to 3000 bp. All scorable bands were polymorphic in nature and none of the primers used produced monomorphic bands, indicating a high level of genetic variation in this grass. The accessions were found to be clustered into eight major groups through the unweighted pair-group method with arithmetic averages. Genetic similarity coefficients (GSC) among the 55 accessions ranged from 0.52 to 0.95. The results clearly indicate that a high level of variation exists in Cynodon accessions. This study shows that the ISSR technique is a reliable tool for differentiating Cynodon accessions and for determining the genetic relationships among them
机译：使用简单序列间重复序列（ISSR）标记估计了55个犬牙龙的遗传变异。本研究中使用的植物材料来自17个国家。用14个引物产生了总共236个ISSR片段。片段大小在200到3000 bp之间。所有可评分的条带本质上都是多态性的，使用的引物均未产生单态性条带，表明该草中的遗传变异水平很高。通过具有算术平均值的非加权对组方法，将这些种质聚类为八个主要组。 55个种质之间的遗传相似性系数（GSC）介于0.52至0.95之间。结果清楚地表明，犬牙根种质中存在高水平的变异。这项研究表明，ISSR技术是区分犬齿种质和确定它们之间遗传关系的可靠工具。

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