...
首页> 外文期刊>Fungal Biology Reviews >Progress in molecular and morphological taxon discovery in Fungi and options for formal classification of environmental sequences
【24h】

Progress in molecular and morphological taxon discovery in Fungi and options for formal classification of environmental sequences

机译:真菌中分子和形态分类群发现的进展以及对环境序列进行正式分类的选择

获取原文
获取原文并翻译 | 示例
           

摘要

Fungal taxonomy seeks to discover, describe, and classify all species of Fungi and provide tools for their identification. About 100,000 fungal species have been described so far, but it has been estimated that there may be from 1.5 to 5.1 million extant fungal species. Over the last decade, about 1200 new species of Fungi have been described in each year. At that rate, it may take up to 4000 y to describe all species of Fungi using current specimen-based approaches. At the same time, the number of molecular operational taxonomic units (MOTUs) discovered in ecological surveys has been increasing dramatically. We analyzed ribosomal RNA internal transcribed spacer (ITS) sequences in the GenBank nucleotide database and classified them as "environmental" or "specimen-based". We obtained 91,225 sequences, of which 30,217 (33 %) were of environmental origin. Clustering at an average 93 % identity in extracted ITS1 and ITS2 sequences yielded 16,969 clusters, including 6230 (37 %) clusters with only environmental sequences, and 2223 (13 %) clusters with both environmental and specimen-based sequences. In 2008 and 2009, the number of purely environmental clusters deposited in GenBank exceeded the number of species described based on specimens, and this does not include the huge number of unnamed MOTUs discovered in pyrosequencing studies. To enable communication about fungal diversity, there is a pressing need to develop classification systems based on environmental sequences. Assigning Latin binomials to MOTUs would promote their integration with specimen-based taxonomic data-bases, whereas the use of numerical codes for MOTUs would perpetuate a disconnect with the taxonomic literature. MOTUs could be formally named under the existing International Code of Botanical Nomenclature if the concept of a nomenclatural type was expanded to include environmental samples or illustrations of sequence chromatograms (or alignments). Alternatively, a "candidate species" category could be created for Fungi, based on the candidatus taxon status employed by microbiologists
机译:真菌分类法旨在发现,描述和分类所有真菌种类,并提供对其进行识别的工具。迄今为止,已经描述了约100,000种真菌,但据估计可能存在1.5至510万种真菌。在过去十年中,每年描述约1200种新真菌。以这种速度,使用当前基于标本的方法描述所有种类的真菌可能需要多达4000年的时间。同时,在生态调查中发现的分子操作分类单位(MOTU)的数量急剧增加。我们分析了GenBank核苷酸数据库中的核糖体RNA内部转录间隔区(ITS)序列,并将其分类为“环境”或“基于标本”。我们获得了91,225个序列,其中30,217个(33%)来自环境。在提取的ITS1和ITS2序列中以平均93%的同一性进行聚类产生了16,969个聚类,包括仅具有环境序列的6230个(37%)聚类和具有基于环境和基于样本的序列的2223个(13%)聚类。在2008年和2009年,存放在GenBank中的纯环境簇的数量超过了根据标本描述的物种数量,这还不包括焦磷酸测序研究中发现的大量未命名的MOTU。为了能够进行有关真菌多样性的交流,迫切需要开发基于环境序列的分类系统。将拉丁语二项式分配给MOTU将促进它们与基于标本的分类数据库的集成,而对MOTU使用数字代码将使分类学文献与世隔绝。如果将命名类型类型的概念扩展到包括环境样本或序列色谱图(或比对)插图,则可以根据现有的《国际植物命名法》正式命名MOTU。或者,可以根据微生物学家采用的念珠菌分类状态为真菌创建“候选物种”类别。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号