...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Functional & integrative genomics >Genomes of parasitic nematodes (Meloidogyne hapla, Meloidogyne incognita, Ascaris suum and Brugia malayi) have a reduced complement of small RNA interference pathway genes: knockdown can reduce host infectivity of M-incognita
【24h】

Genomes of parasitic nematodes (Meloidogyne hapla, Meloidogyne incognita, Ascaris suum and Brugia malayi) have a reduced complement of small RNA interference pathway genes: knockdown can reduce host infectivity of M-incognita

机译：寄生线虫的基因组（Meloidogyne hapla，Meloidogyne incognita，Ascaris suum和Brugia malayi）的小RNA干扰途径基因的补体减少：敲除可以降低M-ogniognita的宿主感染性

获取原文
获取原文并翻译 | 示例
   

获取外文期刊封面封底 >>

       
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The discovery of RNA interference (RNAi) as an endogenous mechanism of gene regulation in a range of eukaryotes has resulted in its extensive use as a tool for functional genomic studies. It is important to study the mechanisms which underlie this phenomenon in different organisms, and in particular to understand details of the effectors that modulate its effectiveness. The aim of this study was to identify and compare genomic sequences encoding genes involved in the RNAi pathway of four parasitic nematodes: the plant parasites Meloidogyne hapla and Meloidogyne incognita and the animal parasites Ascaris suum and Brugia malayi because full genomic sequences were available-in relation to those of the model nematode Caenorhabditis elegans. The data generated was then used to identify some potential targets for control of the root knot nematode, M. incognita. Of the 84 RNAi pathway genes of C. elegans used as model in this study, there was a 42-53 % reduction in the number of effectors in the parasitic nematodes indicating substantial differences in the pathway between species. A gene each from six functional groups of the RNAi pathway of M. incognita was downregulated using in vitro RNAi, and depending on the gene (drh-3, tsn-1, rrf-1, xrn-2, mut-2 and alg-1), subsequent plant infection was reduced by up to 44 % and knockdown of some genes (i.e. drh-3, mut-2) also resulted in abnormal nematode development. The information generated here will contribute to defining targets for more robust nematode control using the RNAi technology.
机译：RNA干扰（RNAi）作为一系列真核生物中基因调控的内源性机制的发现，使其广泛用作功能基因组研究的工具。重要的是研究在不同生物中导致这种现象的机理，尤其是了解调节其有效性的效应子的细节。这项研究的目的是鉴定和比较编码四个寄生线虫RNAi途径中涉及的基因的基因组序列：植物寄生虫Meloidogyne hapla和Meloidogyne incognita以及动物寄生虫Ascaris suum和Brugia malayi，因为可获得完整的基因组序列线虫模型秀丽隐杆线虫的那些。然后将生成的数据用于识别一些潜在的目标，以控制根结线虫，隐孢子虫。在这项研究中用作模型的秀丽隐杆线虫的84个RNAi途径基因中，寄生线虫中效应子的数量减少了42-53％，表明物种之间的途径存在实质性差异。使用体外RNAi下调隐孢子虫RNAi途径的六个功能组的每个基因，具体取决于该基因（drh-3，tsn-1，rrf-1，xrn-2，mut-2和alg- 1），随后的植物感染减少了44％，某些基因（如drh-3，mut-2）的敲除也导致了线虫的异常发育。此处生成的信息将有助于使用RNA干扰技术确定更强大的线虫控制目标。

著录项

相似文献

  • 外文文献
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号