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Another inconsistency in the pedigree of the Oak Ridge wild types of Neurospora crassa

机译:橡树岭野生型Neurospora crassa的谱系中的另一个不一致之处

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A multiple-site molecular dimorphism for the gene for phospholipase C has no known evolutionary basis, yet reveals that Oak Ridge wild types cannot have originated as described in the current best pedigree. This study concerns nucleotide sequences inwild type alleles of the Neurospora crassa gene for phospholipase C (PLC). We have been interested in this gene regarding its role in tip growth. PLC has been proposed to play a role in calcium signal transduction as follows. Under stretch activation inthe plasma membrane, PLC catalyzes the hydrolysis of phosphatidyl inositol 4,5 biphosphate, releasing the second messenger molecules diacylglycerol and inositol-l,4,5-triphosphate (IP3). IP3 binds to a receptor in vacuolar membranes, stimulating the release of Ca~(++) into the cytosol Hence PL C plays a key role in maintaining Ca~(++) gradients in hyphal tip growth (Silvermann-Gavrila and Lew, 2002).
机译:磷脂酶C基因的多位点分子二态性没有已知的进化基础,但揭示了橡树岭野生型不能如当前最佳谱系中所述起源。这项研究涉及磷脂酶C(PLC)的Neurospora crassa基因的野生型等位基因的核苷酸序列。我们一直对该基因在尖端生长中的作用感兴趣。已经提出如下PLC在钙信号转导中起作用。在质膜的拉伸活化作用下,PLC催化磷脂酰肌醇4,5双磷酸酯的水解,释放出第二信使分子二酰基甘油和肌醇-1,4,5-三磷酸酯(IP3)。 IP3与液泡膜上的受体结合,刺激Ca〜(++)释放到细胞质中。因此,PL C在维持菌丝顶端生长中Ca〜(++)梯度中起关键作用(Silvermann-Gavrila和Lew,2002年)。

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