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首页> 外文期刊>Fungal Genetics Newsletter >Two yeast plasmids that confer nourseothricin-dihydrogen sulfate and hygromycin B resistance in Neurospora crassa and Crypho nectria parasitica
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Two yeast plasmids that confer nourseothricin-dihydrogen sulfate and hygromycin B resistance in Neurospora crassa and Crypho nectria parasitica

机译：两个酵母质粒赋予神经孢菌素-二硫酸硫酸盐和潮霉素B抗性

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摘要

Two plasmids that were previously used with yeast, pRS4 IN and pRS41H, were found to confer clonN AT and hygromycin B resistance, respectively, in the filamentous fungi Neurospora crassa and Cryphonectriaparasitica. These plasmids are suitable for routine cloning and for use in forcing heterokaryons and are available through the FGSC. Identification of novel resistance markers is important to further our ability to manipulate and characterize genetic elements in filamentous fungi. Hygromycin B (hygB)inhibits protein translation and has been used extensively as a selectable marker in filamentous fungi (Rao et al,, 1985). Alternative, affordable selectable markers are desirable for various applications, including for forcing heterokaryons. Previously, nourseothricin-dihydrogen sulfate (clonNAT) has been used as a selectable marker in fungi (Kiick and Hoff, 2006). This aminoglycoside binds to ribosomes and results in inhibition and errors in protein synthesis (Cundliffe, 1989). Here we report that two vectors, pRS41N (clonNAT resistance) and pRS41H (hygB resistance), that were previously characterized in S. cerevisiae but not in filamentous fungi, are appropriate transformation vectors and suitable for forcing heterokaryons in Neurospora crassa andCryphonectria parasitica.
机译：发现以前与酵母一起使用的两个质粒pRS4 IN和pRS41H在丝状真菌Neurospora crassa和Cryphonectriaparasitica中分别赋予了clonN AT和潮霉素B抗性。这些质粒适用于常规克隆并用于强迫异核体，可通过FGSC获得。鉴定新的抗性标记对提高我们操纵和鉴定丝状真菌遗传元件的能力很重要。潮霉素B（hygB）抑制蛋白质翻译，并已广泛用作丝状真菌中的选择标记（Rao等，1985）。替代的，负担得起的选择标记对于各种应用是合乎需要的，包括用于强迫异核体。以前，神经柔丝菌素二硫酸氢盐（clonNAT）已被用作真菌中的选择性标记（Kiick and Hoff，2006）。这种氨基糖苷与核糖体结合，导致蛋白质合成受到抑制和错误（Cundliffe，1989）。在这里我们报道了两个载体pRS41N（clonNAT抗性）和pRS41H（hygB抗性）以前在酿酒酵母中表征，但在丝状真菌中没有表征，它们是合适的转化载体，适合于在拟南芥和拟南芥中强迫异核体。

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