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首页> 外文期刊>Canadian Journal of Animal Science >Use of thermal imaging and the in situ technique to assess the impact of an inoculant with feruloyl esterase activity on the aerobic stability and digestibility of barley silage.
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Use of thermal imaging and the in situ technique to assess the impact of an inoculant with feruloyl esterase activity on the aerobic stability and digestibility of barley silage.

机译：使用热成像和原位技术评估具有阿魏酸酯酶活性的孕育剂对大麦青贮饲料有氧稳定性和消化率的影响。

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摘要

Feruloyl esters limit the digestibility of forages, a constraint that may be overcome if additives with feruloyl esterase (FE) activity are applied to forage. This study determined if a FE-producing strain of Lactobacillus buchneri LN4017 in combination with Lactobacillus plantarum LP7109 and Lactobacillus casei LC3200 improves fermentation, aerobic stability and in situ fibre disappearance of whole-crop barley silage. Barley forage (350 g kg -1 DM) was either uninoculated (UN) or inoculated (1.3*10 5 CFU g -1 fresh forage; IN) and ensiled in Ag-Bag silos. Bags were opened after 90 d of ensiling. Samples were collected on day 95, 123 and 175 for determination of fermentation characteristics. Surface temperature of the face of each bag was measured over 3 d using a thermal infrared camera. Ruminal in situ DM and NDF disappearance (aNDFD) of silage and rumen fermentation patterns were measured using three ruminally cannulated heifers. The L. buchneri-treated silage possessed a higher acetic acid concentration than UN (48.8 vs.18.4 g kg -1 DM). The temperature at the face of IN was lower than UN immediately upon exposure and after 3 d of aerobic exposure. Inoculation increased ( P=0.01) 24-h and 48-h aNDFD with a trend towards a glucogenic pattern of ruminal fermentation in heifer compared with UN silage. There was evidence that the FE-producing inoculant improved aerobic stability and aNDFD of whole-crop barley silage.
机译：阿魏酸酯限制了草料的消化率，如果将具有阿魏酸酯酶（FE）活性的添加剂应用于草料，则可以克服这一限制。这项研究确定了产FE的布氏乳杆菌LN4017与植物乳杆菌LP7109和干酪乳杆菌LC3200的结合是否能改善全麦大麦青贮饲料的发酵，有氧稳定性和原位纤维消失。将大麦草料（350 g kg -1 DM）未接种（UN）或接种（1.3 * 10 5 CFU g -1新鲜草料; IN），然后将其青贮在Ag-Bag筒仓中。装盘90天后将袋子打开。在第95、123和175天收集样品以确定发酵特性。使用红外热像仪在3天内测量了每个袋子表面的表面温度。青贮饲料和瘤胃发酵模式的瘤胃原位DM和NDF消失（aNDFD）使用三个瘤胃插管小母牛进行了测定。经布氏乳杆菌处理的青贮饲料具有比联合国更高的乙酸浓度（48.8比18.4 g kg -1 DM）。暴露后立即和有氧暴露3 d后，IN表面的温度均低于UN。与联合国青贮饲料相比，接种增加了24小时和48小时的aNDFD（P = 0.01），并具有小母牛瘤胃发酵的生糖模式。有证据表明，产生FE的孕育剂改善了整粒大麦青贮饲料的有氧稳定性和nNDFD。

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