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Skin regeneration using duck's feet derived collagen and poly(vinyl alcohol) scaffold

机译:使用鸭脚衍生的胶原蛋白和聚乙烯醇支架进行皮肤再生

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摘要

In this study, we have designed scaffold by mixing collagen extracted from the duck's flippers with poly(vinyl alcohol) (DC/PVA) via freeze-thawing and the as-prepared scaffold were evaluated for skin regeneration. The physical and chemical properties of the scaffolds were characterized using FTIR, SEM, compressive strength, degree of swelling, etc. The in vitro behavior (cell proliferation) was examined after cultured with fibroblasts through the 3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyltetrazolium bromide (MTT) assay. The in vivo results demonstrated that the scaffolds implanted in rat induced full-thickness defect, confirmed by histological staining. Encompassing all results, the DC/PVA scaffolds can be anticipated as efficient platforms for skin regeneration facilitating required cell adhesion, growth and proliferation.
机译:在这项研究中,我们设计了支架,方法是通过冻融将从鸭脚板提取的胶原蛋白与聚乙烯醇(DC / PVA)混合,并评估制备好的支架的皮肤再生能力。使用FTIR,SEM,抗压强度,溶胀度等表征支架的物理和化学性质。在成纤维细胞中通过3-(4,5-二甲基噻唑-2)培养后检查了体外行为(细胞增殖)。 -yl)-2,5溴化二苯基四唑(MTT)分析。体内结果表明,通过组织学染色证实,植入大鼠的支架诱导全厚度缺陷。涵盖所有结果,DC / PVA支架有望成为皮肤再生的有效平台,促进所需的细胞粘附,生长和增殖。

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