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首页> 外文期刊>Canadian Journal of Animal Science >Identification of novel variants of interferon-tau gene in Bighorn sheep ( Ovis canadensis mexicana), Pelibuey sheep ( Ovis aries) and its expression in hybrid blastocysts ( Ovis canadensis * ovis aries).
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Identification of novel variants of interferon-tau gene in Bighorn sheep ( Ovis canadensis mexicana), Pelibuey sheep ( Ovis aries) and its expression in hybrid blastocysts ( Ovis canadensis * ovis aries).

机译：在大角羊（Ovis canadensis mexicana），Pelibuey绵羊（Ovis aries）中鉴定干扰素-tau基因的新变体，并在杂交胚泡（Ovis canadensis * ovis aries）中表达。

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摘要

The major signal for maternal recognition of pregnancy (MRP) in ruminants is interferon-tau (IFNT). Four novel sequences of the IFNT gene were isolated from genomic DNA obtained from blood samples of Bighorn, Pelibuey or the hybrid ewes. The deduced amino acid sequence of the hybrid ewe showed 100% identity with one Bighorn sheep IFNT sequence, suggesting that this sequence was inherited from Bighorn sheep. The high degree of identity between Pelibuey and Bighorn sheep sequences could be a possible explanation for the successful MRP in some Bighorn hybrid pregnancies. In vivo and in vitro domestic sheep ( Ovis aries) and hybrid sheep ( Ovis aries * Ovis canadensis) blastocysts were obtained, and IFNT expression was measured by semiquantitative RT-PCR. In vivo and in vitro hybrid blastocysts showed lower expression of IFNT when compared with domestic blastocysts. In conclusion this study showed that there exists an effect of hybridization on IFNT expression and this can affect the MRP.
机译：反刍动物中母体对怀孕的识别（MRP）的主要信号是干扰素-tau（IFNT）。从从比格霍恩，Pelibuey或杂种母羊的血液样品中获得的基因组DNA中分离出IFNT基因的四个新序列。推导的杂种羊氨基酸序列与一只大角羊IFNτ序列具有100％的同一性，表明该序列是从大角羊继承的。 Pelibuey和Bighorn绵羊序列之间的高度同一性可能是某些Bighorn杂交妊娠成功MRP的可能解释。获得体内和体外的家养绵羊（Ovis aries）和杂种绵羊（Ovis aries * Ovis canadensis）胚泡，并通过半定量RT-PCR测定IFNτ的表达。与国内囊胚相比，体内和体外杂种囊胚显示出较低的IFNτ表达。总之，这项研究表明，杂交对IFNτ表达有影响，并且可以影响MRP。

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