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Variation in Hipposideros pratti in China based on morphology and mitochondrial genes

机译:基于形态学和线粒体基因的中国沙棘的变异

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Hipposideros pratti is mainly distributed in mainland China. Many geographical barriers, which might cause different populations ofH. pratti to appear differentiation, exist in its distribution areas. Whether the differentiation having reached the levels for subspecies classification requires further study. Currently, there is not a comprehensive study for the external measurements and molecular sequences of H. pratti. The taxonomic status of H. pratti subspecies remains uncertain. To explore the differences and taxonomic status of H. pratti, we conducted a series of surveys on bats in four different areas of China from 2012 to 2015. We performed multivariate morphometric analyses using 16 external and 25 skull measurements and analysed sequence data of two mitochondrial genes (Cytb and COI). Scatter plots indicated that both external and cranial measurements of samples from the four geographical regions separated into two groups: specimens from Western mountain and plateau subregion in Central China (CW) and Southwest mountainous subregion in Southwest China (WS) gathered into a CW-WS group, and specimens from Eastern hilly and plain subregion in Central China (CE) and Min-Guang coastal subregion in Southern China (SM) gathered into a CE-SM group. The divergence between the CW-WS and CE-SM groups for the Cytb and COI genes were 1.6-2.4% and 1.9-2.3%, respectively, which reached the levels required for identifying subspecies classification according to previous studies. Moreover, in the phylogenetic tree based on Cytb and COI sequences, specimens from the regions CW and WS formed a Glade, and specimens from the regions CE and SM formed another Glade. Both the morphological and molecular results support the conclusion that H. pratti from the CW-WS group represents H. p. pratti, and the CE-SM populations should be termed H. p. sinicus. (C) 2016 Deutsche Gesellschaft fur Saugetierkunde. Published by Elsevier GmbH. All rights reserved.
机译:沙棘主要分布于中国大陆。许多地理障碍,可能导致不同的H种群。普拉蒂出现分化,存在于其分布区域。分化是否已经达到亚种分类的水平需要进一步研究。目前,尚未对H.pratti的外部测量和分子序列进行全面的研究。普拉提亚种的分类学地位仍然不确定。为了探讨H. pratti的差异和分类学状况,我们在2012年至2015年对中国四个不同地区的蝙蝠进行了一系列调查。我们使用16个外部和25个头骨测量值进行了多变量形态分析,并分析了两个线粒体的序列数据基因(Cytb和COI)。散点图表明,将来自四个地理区域的样本的外部和颅骨测量都分为两组:来自中国中部西部山区和高原子区域的样本以及中国西南地区西南山地样本的样本都收集到了CW-WS中来自中国中部东部丘陵和平原分区域(CE)和中国南部闽广沿海次区域(SM)的标本收集到一个CE-SM组。 Cytb和COI基因的CW-WS和CE-SM组之间的差异分别为1.6-2.4%和1.9-2.3%,达到了根据先前研究确定亚种分类所需的水平。此外,在基于Cytb和COI序列的系统发育树中,来自CW和WS区域的标本形成了Glade,来自CE和SM区域的标本形成了另一个Glade。形态学和分子学结果均支持来自CW-WS组的H. pratti代表H. p。的结论。 pratti,CE-SM人群应称为H. p.。鼻窦。 (C)2016德国皮草拍卖行(Saugetierkunde)。由Elsevier GmbH发布。版权所有。

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