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首页> 外文期刊>Canadian Journal of Animal Science >Molecular cloning of the duck MyoG and MRF4 genes coding region sequence and their differential expression patterns in the breast and leg muscle during fetal development
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Molecular cloning of the duck MyoG and MRF4 genes coding region sequence and their differential expression patterns in the breast and leg muscle during fetal development

机译：鸭MyoG和MRF4基因编码区序列的分子克隆及其在胎儿发育过程中乳房和腿部肌肉的差异表达模式。

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Molecular cloning of the duck MyoG and MRF4 genes CDS and their differential expression patterns in the breast and leg muscle during fetal development. Can. J. Anim. Sci. 90: 179-188. The MyoG and MRF4 genes encode for transcription factors that play key roles in muscle formation during embryonic and fetal development. Due to the unique functions of these two genes, they have a specific expression pattern in the same muscle tissues. In order to investigate the different developmental expression patterns of the two genes, the coding region sequences of duck MyoG and MRF4 were cloned and their mRNA expression patterns in the duck breast and leg muscles were measured using real-time quantitative polymerase chain reaction (qPCR). The duck MyoG and MRF4 both have a basic helix-loop-helix (bHLH) domain with high homology to several other species. In addition, there is a low homology region at the carboxy-terminus of these two proteins. The mRNA expression of MyoG reaches a peak at embryonic day 14 (E14d) in both the breast muscle and the leg muscle of duck. The peak mRNA expression of MRF4 appears at E14d in the breast muscle and at E18d in the leg muscle. The expression patterns of MyoG and MRF4 were different between the breast muscle and the leg muscle of duck. In the fetal duck, the leg muscle develops earlier than the breast muscle, which may better accommodate the newborn duck to a new environment postnatally.
机译：鸭MyoG和MRF4基因CDS的分子克隆及其在胎儿发育过程中在乳房和腿部肌肉中的差异表达模式。能够。 J.动漫科学90：179-188。 MyoG和MRF4基因编码在胚胎和胎儿发育过程中在肌肉形成中起关键作用的转录因子。由于这两个基因的独特功能，它们在相同的肌肉组织中具有特定的表达模式。为了研究这两个基因的不同发育表达方式，克隆了鸭MyoG和MRF4的编码区序列，并使用实时定量聚合酶链反应（qPCR）测定了它们在鸭胸和腿部肌肉中的mRNA表达方式。 。鸭MyoG和MRF4都具有基本的螺旋-环-螺旋（bHLH）结构域，与其他几个物种具有高度同源性。此外，在这两种蛋白质的羧基末端有一个低同源性区域。鸭的胸部和腿部肌肉中，MyoG的mRNA表达在胚胎第14天（E14d）达到峰值。 MRF4的mRNA峰值表达出现在胸部肌肉的E14d和腿部肌肉的E18d。鸭的胸部肌肉和腿部肌肉的MyoG和MRF4的表达方式不同。在胎鸭中，腿部肌肉比胸部肌肉发育得早，这可能会更好地使新生鸭在出生后适应新的环境。

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