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首页> 外文期刊>Expert review of proteomics >Analysis of organelles within the nervous system: impact on brain and organelle functions.
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Analysis of organelles within the nervous system: impact on brain and organelle functions.

机译：分析神经系统内的细胞器：对大脑和细胞器功能的影响。

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Currently, neuroproteomic approaches aimed at the profiling of total brain areas generally mirror the expression of the most abundant proteins, but fail to uncover less abundant proteins. By contrast, the focus on typical brain subproteomes, (e.g., synaptic vesicles, synaptic terminal membranes or the postsynaptic density), may give a more specific insight into brain function. Subproteomes are accessible via several strategies, including subcellular fractionation or affinity-based pull-down approaches. Combined with mass spectrometric quantification approaches, subcellular proteomics is expected to reveal differences in the protein constitution of related cellular organelles. Focusing on novel functions and mechanistic models, we review recent data on the analysis of brain-derived organelles and subproteomes, including presynaptic termini, synaptic vesicles, neuronal plasma membranes, postsynaptic density and neuromelanin granules, which were identified as novel lysosome-related organelles within the human brain.
机译：当前，针对整个大脑区域轮廓的神经蛋白质组学方法通常反映了最丰富的蛋白质的表达，但未能发现较丰富的蛋白质。相比之下，对典型脑子蛋白质组（例如，突触小泡，突触终末膜或突触后密度）的关注可能会提供对脑功能的更具体的见识。可以通过多种策略访问子蛋白质组，包括亚细胞分级分离或基于亲和力的下拉方法。结合质谱定量方法，亚细胞蛋白质组学有望揭示相关细胞器蛋白质组成的差异。着眼于新颖的功能和机制模型，我们回顾了有关脑源性细胞器和子蛋白质组分析的最新数据，包括突触前末端，突触小泡，神经元质膜，突触后密度和神经黑色素颗粒，这些被鉴定为与内源性溶酶体有关的新型细胞器人脑。

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