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首页> 外文期刊>Frontiers in bioscience: a journal and virtual library >A single vaccination with attenuated SIVmac 239 via the tonsillar route confers partial protection against challenge with SIVmac 251 at a distant mucosal site, the rectum
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A single vaccination with attenuated SIVmac 239 via the tonsillar route confers partial protection against challenge with SIVmac 251 at a distant mucosal site, the rectum

机译：通过扁桃体途径对减毒SIVmac 239进行的单次疫苗接种可在较远的粘膜部位（直肠）针对SIVmac 251的攻击提供部分保护

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摘要

Elucidating the mechanisms that protect monkeys previously immunized with attenuated SIV (SIVDeltanef) against challenge infection with pathogenic virus may reveal new strategies for the development of an effective HIV vaccine. Here we show that a single atraumatic application of SIVDeltanef to the tonsils of four rhesus macaques conferred protection against SIVmac251 applied intrarectally 26 weeks later. While this protection was not complete, i.e., challenge virus could be isolated from all immunized animals, it was reflected by significantly lower viral loads in the blood (weeks 2-16 after challenge, p<0.01) and considerably lower loads in lymphoid organs, and more stable peripheral CD4 counts in a proportion of the immunized animals as compared to four non-immunized, SIVmac251 -infected control monkeys. SIV-specific humoral as well as systemic and mucosal T cell responses were detected in the immunized animals, but there was no correlation between their magnitude of expression and the level of protection. Analyses of leukocyte subsets in these animals at necropsy (24 weeks after challenge) did not reveal a significantly enhanced proportion of gamma/delta T cells in the tissues of protected monkeys. Therefore, tonsillar application of attenuated SIV induces protection in some animals against a superinfection with wild-type SIV distant at a distant mucosal site.
机译：阐明保护先前用减毒SIV（SIVDeltanef）免疫的猴子免受病原病毒攻击感染的机制可能揭示了开发有效HIV疫苗的新策略。在这里，我们显示了对四个恒河猴的扁桃体进行单次无创应用SIVDeltanef可以防止26周后直肠内应用SIVmac251。虽然这种保护措施还不完善，也就是说，可以从所有免疫动物中分离出攻击病毒，但血液中的病毒载量显着降低（攻击后2-16周，p <0.01），淋巴器官的载量显着降低，反映了这一点，与四只未免疫的SIVmac251感染的对照猴子相比，在一定比例的免疫动物中外周CD4计数更稳定。在免疫动物中检测到SIV特异性的体液以及全身和粘膜T细胞反应，但它们的表达量和保护水平之间没有相关性。尸检（攻击后24周）中这些动物中的白细胞亚群的分析未显示出受保护的猴子组织中γ/δT细胞的比例显着增加。因此，扁桃体减毒SIV的应用在一些动物中诱导保护免受在远处的粘膜部位远处的野生型SIV的过度感染。

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