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首页> 外文期刊>Frontiers in bioscience: a journal and virtual library >Identification of ICIS-1, a new protein involved in cilia stability
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Identification of ICIS-1, a new protein involved in cilia stability

机译：鉴定ICIS-1，一种参与纤毛稳定性的新蛋白

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摘要

Cilia are specialized organelles that exert critical functions in numerous organisms, including that of cell motility, fluid transport and protozoan locomotion. Ciliary architecture and function strictly depend on basal body formation, migration and axoneme elongation. Numerous ultrastructural studies have been undertaken in different species to elucidate the process of ciliogenesis. Recent analyses have led to identification of genes specifically expressed in ciliated organisms, but most proteins involved in ciliogenesis remain uncharacterized. Using human nasal epithelial cells capable of ciliary differentiation in vitro, differential display was carried out to identify new proteins associated with ciliogenesis. We isolated a new gene, ICIS-1 (Involved in Cilia Stability-1), upregulated during mucociliary differentiation. This gene is localized within the TGF-betal promoter and is ubiquitously expressed in human tissues. Functional analyses of gene expression inhibition by RNA interference in Paramecium tetraurelia indicated that the ICIS-1 homologue interfered with cilia stability or formation. These findings demonstrate that ICIS-1 is a new protein associated with ciliated cells and potentially related to cilia stability.
机译：纤毛是在许多生物体中发挥关键功能的专门细胞器，包括细胞运动，液体运输和原生动物的运动。睫状结构和功能严格取决于基础体的形成，迁移和轴突伸长。已经在不同物种中进行了许多超结构研究，以阐明纤毛发生的过程。最近的分析已导致鉴定出在纤毛生物中特异性表达的基因，但大多数与纤毛发生有关的蛋白质仍未鉴定。使用能够在体外进行睫状细胞分化的人鼻上皮细胞，进行了差异展示以鉴定与睫毛发生有关的新蛋白。我们分离出一个新基因，ICIS-1（参与纤毛稳定性-1），在粘膜纤毛分化过程中被上调。该基因位于TGF-β1启动子内，并在人体组织中普遍表达。功能分析对四氢草履虫中RNA干扰的基因表达抑制作用表明，ICIS-1同源物干扰了纤毛的稳定性或形成。这些发现表明，ICIS-1是与纤毛细胞相关的新蛋白，并且可能与纤毛稳定性有关。

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