• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Marine biotechnology >Molecular genetic identification tools for three commercially cultured oysters (Crassostrea belcheri, Crassostrea iredalei, and saccostrea cucullata) in Thailand
【24h】

Molecular genetic identification tools for three commercially cultured oysters (Crassostrea belcheri, Crassostrea iredalei, and saccostrea cucullata) in Thailand

机译：泰国三种商业养殖牡蛎（Crassostrea belcheri，Crassostrea iredalei和saccostrea cucullata）的分子遗传识别工具

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Molecular genetic keys for identification of 3 commercially cultured oysters (Crassostrea belcheri, Crassostrea iredalei, and Saccostrea cucullata) in Thailand were developed based on restriction analysis of 18S ribosomal DNA and cytochrome oxidase subunit I (COI). Digestion of the amplified 18S rDNA with Hinf I unambiguously differentiated Crassostrea oysters from Saccostrea oysters and Striostrea (Parastriostrea) mytiloides. In addition, species-specific restriction fragment length polymorphism patterns of C. belcheri, C. iredalei, and S. cucullata were consistently observed when the gel-eluted COI was digested with Mbo I and Dde I. Thirty composite haplotypes were observed across all individuals. Species-specific composite haplotypes were found in C. belcheri (AAAA and AAAB), C. iredalei (AABC and AABU), and S. cucullata (BBCD and BBCE), respectively. The most common composite haplotype of COI in C. belcheri (AAAA), C. iredalei (AABC), and S. cucullata (BBCD) was amplified, cloned, and sequenced. Detection of C. belcheri and C. iredalei based on polymerase chain reaction was further developed using more specific primers (HCO2198 and R372) followed by digestion of a 372-bp product with Mbo I.
机译：基于对18S核糖体DNA和细胞色素氧化酶亚基（COI）的限制性分析，开发了用于鉴定泰国3种商业养殖牡蛎（Crassostrea belcheri，Crassostrea iredalei和Saccostrea cucullata）的分子遗传钥匙。用Hinf I对扩增的18S rDNA进行的消化可将角s属牡蛎与S形牡蛎和三角藻（Parastriostrea）淀粉样蛋白明确区分开。此外，当用Mbo I和Dde I消化凝胶洗脱的COI时，始终观察到C. belcheri，C。iredalei和S. cucullata的物种特异性限制性片段长度多态性模式。在所有个体中观察到30种复合单倍型。物种特有的复合单倍型分别存在于Bel。chercheri（AAAA和AAAB），iredalei。（AABC和AABU）和S. cucullata（BBCD和BBCE）中。扩增，克隆并测序了在BELC。belcheri（AAAA），iredale C. iredalei（AABC）和S. cucullata（BBCD）中最常见的COI复合单倍型。利用聚合酶链式反应进一步检测belcheri和I. daledalei。使用更特异性的引物（HCO2198和R372），然后用Mbo I消化372bp的产物。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号