• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Marine genomics >Characterization of a mitochondrial ORF from the gender-associated mtDNAs of Mytilus spp. (Bivalvia: Mytilidae): Identification of the 'missing' ATPase 8 gene
【24h】

Characterization of a mitochondrial ORF from the gender-associated mtDNAs of Mytilus spp. (Bivalvia: Mytilidae): Identification of the 'missing' ATPase 8 gene

机译：从Mytilus spp的与性别相关的mtDNA线粒体ORF的特征。 （双壳纲：Mytilidae）：鉴定“缺失的” ATPase 8基因

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Bivalve species are characterized by extraordinary variability in terms of mitochondrial (mt) genome size, gene arrangement and tRNA gene number. Many species are thought to lack the mitochondrial protein-coding gene atp8. Of these species, the Mytilidae appears to be the only known taxon with doubly uniparental inheritance of mtDNA that does not possess the atp8 gene. This raises the question as to whether mytilids have completely lost the ATP8 protein, whether the gene has been transferred to the nucleus or whether they possess a highly modified version of the gene/protein that has led to its lack of annotation. In the present study, we re-investigated all complete (or nearly complete) F and M mytilid mt genomes previously sequenced for the presence of conserved open reading frames (ORFs) that might code for ATP8 and/or have other functional importance in these bivalves. We also revised the annotations of all available complete mitochondrial genomes of bivalves and nematodes that are thought to lack atp8 in an attempt to detect it. Our results indicate that a novel mytilid ORF of significant length (i.e., the ORF is >85 amino acids in length), with complete start and stop codons, is a candidate for the atp8 gene: (1) it possesses a pattern of evolution expected for a protein-coding gene evolving under purifying selection (i.e., the 3rd > 1st > 2nd codon pattern of evolution), (2) it is actively transcribed in Mytilus species, (3) it has one predicted transmembrane helix (as do other metazoan ATP8 proteins), (4) it has conserved functional motifs and (5), comparisons of its amino acid sequence with ATP8 sequences of other molluscan or bivalve species reveal similar hydropathy profiles. Furthermore, our revised annotations also confirmed the mt presence of atp8 in almost all bivalve species and in one nematode species. Our results thus support recognizing the presence of ATPase 8 in most bivalves mt genomes (if not all) rather than the continued characterization of these genomes as lacking this gene.
机译：双壳类物种的特征在于线粒体（mt）基因组大小，基因排列和tRNA基因数目方面的非凡变异性。人们认为许多物种缺乏线粒体蛋白编码基因atp8。在这些物种中，Mytilidae似乎是唯一已知的具有双重单亲遗传的mtDNA的分类单元，它不具有atp8基因。这就提出了一个问题，即，甲壳类动物是否已完全丧失了ATP8蛋白，该基因是否已转移到细胞核中，或者它们是否具有导致其缺乏注释的基因/蛋白的高度修饰形式。在本研究中，我们重新研究了先前测序的所有完整（或近乎完整）的F和M Mytilid mt基因组，这些保守的开放阅读框（ORF）的存在可能编码ATP8和/或在这些双壳类动物中具有其他功能重要性。我们还修订了被认为缺乏atp8的双壳类和线虫所有可用的完整线粒体基因组的注释，以试图检测它。我们的结果表明，具有完整的起始密码子和终止密码子的，长度显着（即，ORF长度大于85个氨基酸）的新型Mytilid ORF是atp8基因的候选者：（1）它具有预期的进化模式对于在纯化选择下进化的蛋白质编码基因（即进化的第三个> 1st>第二个密码子模式），（2）它在Mytilus物种中活跃转录，（3）它具有一个预测的跨膜螺旋（其他后生动物也是如此） ATP8蛋白），（4）具有保守的功能基序，（5），其氨基酸序列与其他软体动物或双壳类物种的ATP8序列的比较显示出相似的亲水性。此外，我们修订的注释还证实了atp8在几乎所有双壳类和线虫中均存在mt。因此，我们的结果支持识别大多数双壳类mt基因组（如果不是全部）中ATPase 8的存在，而不是将这些基因组继续表征为缺少该基因。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号