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首页> 外文期刊>Marine genomics >Discovery of germline-related genes in Cephalochordate amphioxus: A genome wide survey using genome annotation and transcriptome data
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Discovery of germline-related genes in Cephalochordate amphioxus: A genome wide survey using genome annotation and transcriptome data

机译：在头孢金合phi中发现与种系相关的基因：使用基因组注释和转录组数据进行全基因组调查

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The generation of germline cells is a critical process in the reproduction of multicellular organisms. Studies in animal models have identified a common repertoire of genes that play essential roles in primordial germ cell (PGC) formation. However, comparative studies also indicate that the timing and regulation of this core genetic program vary considerably in different animals, raising the intriguing questions regarding the evolution of PGC developmental mechanisms in metazoans. Cephalochordates (commonly called amphioxus or lancelets) represent one of the invertebrate chordate groups and can provide important information about the evolution of developmental mechanisms in the chordate lineage. In this study, we used genome and transcriptome data to identify germline-related genes in two distantly related cephalochordate species, Branchiostoma floridae and Asymmetron lucayanum. Branchiostoma and Asymmetron diverged more than 120 MYA, and the most conspicuous difference between them is their gonadal morphology. We used important germline developmental genes in several model animals to search the amphioxus genome and transcriptome dataset for conserved homologs. We also annotated the assembled transcriptome data using Gene Ontology (GO) terms to facilitate the discovery of putative genes associated with germ cell development and reproductive functions in amphioxus. We further confirmed the expression of 14 genes in developing oocytes or mature eggs using whole mount in situ hybridization, suggesting their potential functions in amphioxus germ cell development. The results of this global survey provide a useful resource for testing potential functions of candidate germline-related genes in cephalochordates and for investigating differences in gonad developmental mechanisms between Branchiostoma and Asymmetron species. (C) 2015 Elsevier B.V. All rights reserved.
机译：种系细胞的产生是多细胞生物繁殖中的关键过程。动物模型研究确定了在原始生殖细胞（PGC）形成中起重要作用的常见基因。但是，比较研究还表明，不同动物中这一核心遗传程序的时间安排和调控方式存在很大差异，这引发了有关后生动物中PGC发育机制演变的有趣问题。头足类动物（通常称为两栖类或长矛类动物）代表无脊椎动物的碳酸盐类之一，可以提供有关碳酸盐类谱系发育机制进化的重要信息。在这项研究中，我们使用基因组和转录组数据来鉴定两个远缘相关头孢菌种佛罗里达分枝杆菌和Aymmetron lucayanum中与种系相关的基因。支气管造口和Asymmetron的差异超过120 MYA，它们之间最明显的区别是它们的性腺形态。我们在几个模型动物中使用了重要的种系发育基因，以搜寻两栖类基因组和转录组数据集来寻找保守的同源物。我们还使用基因本体论（GO）术语注释了组装的转录组数据，以促进发现与两性生殖中生殖细胞发育和生殖功能相关的推定基因。我们进一步证实了使用整装原位杂交技术在发育中的卵母细胞或成熟卵中14个基因的表达，表明了它们在文昌鱼生殖细胞发育中的潜在功能。这项全球调查的结果提供了有用的资源，可用于测试头线虫中与候选种系相关的基因的潜在功能，以及调查分支气孔和非对称性物种之间性腺发育机制的差异。 （C）2015 Elsevier B.V.保留所有权利。

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