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首页> 外文期刊>Mass Spectrometry Reviews >ADVANCES ON THE COMPOSITIONAL ANALYSIS OF GLYCOSPHINGOLIPIDS COMBINING THIN-LAYER CHROMATOGRAPHY WITH MASS SPECTROMETRY
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ADVANCES ON THE COMPOSITIONAL ANALYSIS OF GLYCOSPHINGOLIPIDS COMBINING THIN-LAYER CHROMATOGRAPHY WITH MASS SPECTROMETRY

机译：薄层色谱-质谱联用分析糖脂类化合物的研究进展

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Glycosphingolipids (GSLs), composed of a hydrophilic carbohydrate chain and a lipophilic ceramide anchor, play pivotal roles in countless biological processes, including infectious diseases and the development of cancer. Knowledge of the number and sequence of monosaccharides and their anomeric configuration and linkage type, which make up the principal items of the glyco code of biologically active carbohydrate chains, is essential for exploring the function of GSLs. As part of the investigation of the vertebrate glycome, GSL analysis is undergoing rapid expansion owing to the application of novel biochemical and biophysical technologies. Mass spectrometry (MS) takes part in the network of collaborations to further unravel structural and functional aspects within the fascinating world of GSLs with the ultimate aim to better define their role in human health and disease. However, a single-method analytical MS technique without supporting tools is limited yielding only partial structural information. Because of its superior resolving power, robustness, and easy handling, high-performance thin-layer chromatography (TLC) is widely used as an invaluable tool in GSL analysis. The intention of this review is to give an insight into current advances obtained by coupling supplementary techniques such as TLC and mass spectrometry. A retrospective view of the development of this concept and the recent improvements by merging (1) TLC separation of GSLs, (2) their detection with oligosaccharide-specific proteins, and (3) in situ MS analysis of protein-detected GSLs directly on the TLC plate, are provided. The procedure works on a nanogram scale and was successfully applied to the identification of cancer-associated GSLs in several types of human tumors. The combination of these two supplementary techniques opens new doors by delivering specific structural information of trace quantities of GSLs with only limited investment in sample preparation.
机译：糖鞘脂（GSLs）由亲水性碳水化合物链和亲脂性神经酰胺锚定物组成，在无数生物学过程（包括传染病和癌症的发展）中起着关键作用。组成生物活性碳水化合物链糖代码主要项目的单糖的数量和序列及其异头构型和连接类型的知识对于探索GSL的功能至关重要。作为脊椎动物糖蛋白研究的一部分，由于新型生化和生物物理技术的应用，GSL分析正在迅速扩展。质谱（MS）参与了合作网络，以进一步阐明迷人的GSL世界中的结构和功能方面，最终目的是更好地定义它们在人类健康和疾病中的作用。但是，没有支持工具的单方法分析MS技术受到限制，仅产生部分结构信息。高性能薄层色谱（TLC）由于其出色的分离能力，耐用性和易操作性，被广泛用作GSL分析中的宝贵工具。这篇综述的目的在于深入了解通过结合使用诸如TLC和质谱之类的辅助技术所获得的最新进展。通过合并（1）GSL的TLC分离，（2）用寡糖特异性蛋白进行检测以及（3）直接在检测仪上对蛋白质检测到的GSL进行原位MS分析来回顾此概念的发展和最近的改进。提供了TLC板。该程序的工作量为纳克级，已成功应用于多种类型人类肿瘤中与癌症相关的GSL的鉴定。这两种辅助技术的结合通过提供少量痕量GSL的特定结构信息而打开了新的大门，而对样品制备的投资却很少。

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