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首页> 外文期刊>Mass Spectrometry Reviews >ANALYSIS OF VITAMIN D METABOLIC MARKERS BY MASS SPECTROMETRY: CURRENT TECHNIQUES, LIMITATIONS OF THE 'GOLD STANDARD' METHOD, AND ANTICIPATED FUTURE DIRECTIONS
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ANALYSIS OF VITAMIN D METABOLIC MARKERS BY MASS SPECTROMETRY: CURRENT TECHNIQUES, LIMITATIONS OF THE 'GOLD STANDARD' METHOD, AND ANTICIPATED FUTURE DIRECTIONS

机译：质谱法分析维生素D代谢物标记：最新技术，“金标准”方法的局限性和预期的未来方向

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Vitamin D compounds belong to a group of secosteroids, which occur naturally as vitamin D-3 in mammals and D-2 in plants. Vitamin D is vital for bone health but recent studies have shown a much wider role in the pathologies of diseases such as diabetes, cancer, autoimmune, neurodegenerative, mental and cardiovascular diseases. Photosynthesis of vitamin D in the human skin and subsequent hepatic and renal metabolism generate a wide range of transformation products occurring over a large dynamic range spanning from picomolar to nanomolar levels. This necessitates selective and sensitive analytical methods to quantitatively capture these low concentration levels in relevant tissues such as blood. Ideally, vitamin D assessment would be performed using a universal and standardized analytical method available to clinical laboratories that provides reliable and accurate quantitative results for all relevant vitamin D metabolites with sufficiently high throughput. At present, LC-MS/MS assays are the most promising techniques for vitamin D analysis. The present review focuses on developments in mass spectrometry methodologies of the past 12 years. It will highlight detrimental influences of the biological matrix, epimer contributions, pitfalls of specific mass spectrometry data acquisition routines (in particular multiple reaction monitoring, MRM), influence of ionization source, derivatization reactions, inter-laboratory comparisons on precision, accuracy, and application range of vitamin D metabolites. (c) 2013 Wiley Periodicals, Inc. Mass Spec Rev 34: 2-23, 2015.
机译：维生素D化合物属于一组类固醇，它们在哺乳动物中以维生素D-3天然存在，在植物中以D-2天然存在。维生素D对骨骼健康至关重要，但最近的研究表明，在糖尿病，癌症，自身免疫性疾病，神经退行性疾病，精神疾病和心血管疾病等疾病的病理学中，维生素D的作用更为广泛。人体皮肤中维生素D的光合作用以及随后的肝和肾新陈代谢产生了一系列变化产物，这些变化产物发生在从皮摩尔到纳摩尔水平的较大动态范围内。这需要选择性和灵敏的分析方法来定量捕获相关组织（例如血液）中的这些低浓度水平。理想情况下，将使用临床实验室可用的通用和标准化分析方法进行维生素D评估，该方法可为所有相关的维生素D代谢物提供足够高通量的可靠和准确的定量结果。目前，LC-MS / MS分析是最有前途的维生素D分析技术。本综述着重于过去12年中质谱方法学的发展。它将重点介绍生物基质的有害影响，差向异构体的贡献，特定质谱数据采集程序（特别是多反应监测，MRM）的陷阱，电离源的影响，衍生化反应，实验室间比较对精度，准确性和应用的影响范围的维生素D代谢产物。 （c）2013 Wiley Periodicals，Inc.质谱修订版34：2-23，2015。

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