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首页> 外文期刊>Biochemistry >Distinct Biological Activity of Lipopolysaccharides with Different Lipid A Acylation Status from Mutant Strains of Yersinia pestis and Some Members of Genus Psychrobacter
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Distinct Biological Activity of Lipopolysaccharides with Different Lipid A Acylation Status from Mutant Strains of Yersinia pestis and Some Members of Genus Psychrobacter

机译：鼠疫耶尔森氏菌和某些精神杆菌属成员突变株具有不同脂质A酰化状态的脂多糖的独特生物活性

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摘要

Correlation between the chemical structure of lipid A from various Gram-negative bacteria and biological activity of their lipopolysaccharide (LPS) as an agonist of the innate immune receptor Toll-like receptor 4 was investigated. Purified LPS species were quantitatively evaluated by their ability to activate the production of tumor necrosis factor (TNF) by murine bone marrow-derived macrophages in vitro. Wild-type LPS from plague-causing bacteria Yersinia pestis was compared to LPS from mutant strains with defects in acyltransferase genes (lpxM, lpxP) responsible for the attachment of secondary fatty acid residues (12:0 and 16:1) to lipid A. Lipid A of Y. pestis double Delta lpxM/Delta lpxP mutant was found to have the chemical structure that was predicted based on the known functions of the respective acyltransferases. The structures of lipid A from two members of the ancient psychrotrophic bacteria of the genus Psychrobacter were established for the first time, and biological activity of LPS from these bacteria containing lipid A fatty acids with shorter acyl chains (C10-C12) than those in lipid A from LPS of Y. pestis or E. coli (C12-C16) was determined. The data revealed a correlation between the ability of LPS to activate TNF production by bone marrow-derived macrophages with the number and the length of acyl chains within lipid A.
机译：研究了来自多种革兰氏阴性细菌的脂质A的化学结构与其作为先天免疫受体Toll样受体4激动剂的脂多糖（LPS）的生物学活性之间的相关性。纯化的LPS物种通过体外小鼠骨髓衍生的巨噬细胞激活肿瘤坏死因子（TNF）产生的能力进行定量评估。将鼠疫鼠疫耶尔森氏菌的野生型LPS与突变型菌株的LPS进行了比较，该突变菌株的酰基转移酶基因（lpxM，lpxP）有缺陷，负责将次要脂肪酸残基（12：0和16：1）连接到脂质A.发现鼠疫耶尔森氏菌双ΔlpxM/ΔlpxP突变体的脂质A具有基于相应酰基转移酶的已知功能预测的化学结构。首次建立了古老的精神营养细菌属的一种成员的脂质A的结构，这些细菌的脂多糖具有比脂质中短的酰基链（C10-C12）的脂多糖测定了鼠疫耶尔森氏菌或大肠杆菌的脂多糖（C12-C16）的A。数据揭示了LPS激活骨髓巨噬细胞激活TNF产生的能力与脂质A中酰基链的数量和长度之间的相关性。

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