Cloning and functional expression of a chitinase cDNA from the apple leaf miner moth Lithocolletis ringoniella

Fan Xiao-Jun; Mi Yan-Xia; Ren Hui; Zhang Chang; Li Yao; Xian Xiao-Xiao

首页> 外文期刊>Biochemistry >Cloning and functional expression of a chitinase cDNA from the apple leaf miner moth Lithocolletis ringoniella

【24h】

Cloning and functional expression of a chitinase cDNA from the apple leaf miner moth Lithocolletis ringoniella

机译：苹果叶片蛾蛾Licollcolletis ringoniella几丁质酶cDNA的克隆及功能表达

获取原文

获取原文并翻译 | 示例

掌桥外文数据库（机构版） >>

开具论文收录证明 >>

文献代查 >>

页面导航

摘要
著录项
相似文献
相关主题

摘要

Insect chitinase plays essential roles in chitin catabolism involved in digestion and molting during insect development. In the current work, we cloned a chitinase cDNA, LrCht5, from the apple leaf miner moth Lithocolletis ringoniella and characterized its amino acid sequence and protein properties. The L. ringoniella chitinase cDNA was 2136 bp in length with an open reading frame of 1737 bp that encodes a polypeptide of 579 amino acid residues with a predicted molecular mass of 64.4 kDa and pI of 5.49. The catalytic domain has several phosphorylation and glycosylation sites. The recombinant LrCht5 was expressed in Escherichia coli and the Spodoptera frugiperda cell line Sf9, and the LrCht5 expressed in insect cells exhibited chitinolytic activity. LrCht5 was most stable at pH 6.0 and 45A degrees C. This work has potential application in the development of novel and more specific synthetic chitinase inhibitors for use as bioinsecticides.

机译：昆虫几丁质酶在昆虫发育过程中参与消化和蜕皮的几丁质分解代谢中起重要作用。在当前的工作中，我们从苹果叶蛾蛾Licollcolletis ringoniella克隆了几丁质酶cDNA LrCht5，并对其氨基酸序列和蛋白质特性进行了表征。鼠李糖乳杆菌几丁质酶cDNA的长度为2136 bp，开放阅读框为1737 bp，其编码579个氨基酸残基的多肽，预测分子量为64.4 kDa，pI为5.49。催化结构域具有几个磷酸化和糖基化位点。重组LrCht5在大肠杆菌和草地贪夜蛾细胞系Sf9中表达，在昆虫细胞中表达的LrCht5表现出几丁质分解活性。 LrCht5在pH 6.0和45A摄氏度下最稳定。这项工作在开发新型和更特异性的合成几丁质酶抑制剂用作生物杀虫剂方面具有潜在的应用前景。

著录项

来源
《Biochemistry》 |2015年第2期|共9页
作者
Fan Xiao-Jun; Mi Yan-Xia; Ren Hui; Zhang Chang; Li Yao; Xian Xiao-Xiao;
展开▼
作者单位

展开▼
收录信息
原文格式 PDF
正文语种 eng
中图分类生物化学;
关键词
Lithocolletis ringoniella; chitinase; RACE; enzyme activity;

机译：环形石怪;几丁质酶;RACE;酶活性;

相似文献

外文文献
中文文献
专利

1. Cloning and functional expression of a chitinase cDNA from the apple leaf miner moth Lithocolletis ringoniella [J] . Fan Xiao-Jun, Mi Yan-Xia, Ren Hui, Biochemistry . 2015,第2期

机译：苹果叶片蛾蛾Licollcolletis ringoniella几丁质酶cDNA的克隆及功能表达
2. Molecular Cloning and Functional Expression of Chitinase-Encoding cDNA from the Cabbage Moth, Mamestra brassicae [J] . Aron Paek, Hee Yun Park, Seong Eun Jeong Molecules and cells . 2012,第5期

机译：白菜蛾 Mamestra brasicae 几丁质酶编码cDNA的分子克隆和功能表达

3. Cloning and functional expression of an (E,E)- alpha -farnesene synthase cDNA from apple fruit. [J] . Pechous S W, Whitaker B D Acta Horticulturae . 2005,第1期

机译：苹果果实中（E，E）-α-法呢烯合酶cDNA的克隆和功能表达。

4. Clone in vitro expression and functional analysis of the cDNA encoding a putative nucleoside transporter from arabidopsis thaliana [C] . Li Jian, Wang Daowen International conference on engineering and technological sciences;ICETS2000 . 2000

机译：拟南芥核苷转运蛋白编码cDNA的克隆体外表达及功能分析

5. Generation of full-length cDNA clone and functional analysis of leader proteases of Grapevine leafroll-associated virus-2. [D] . Liu, Yu-Ping. 2009

机译：葡萄叶片相关病毒2的全长cDNA克隆的产生和前导蛋白酶的功能分析。

6. Molecular Cloning and Functional Expression of Chitinase-Encoding cDNA from the Cabbage Moth Mamestra brassicae [O] . Aron Paek, Hee Yun Park, Seong Eun Jeong 2012

机译：甘蓝小菜蛾几丁质酶编码cDNA的分子克隆和功能表达

7. Studies on the Mating Behavior of the Apple Leaf Miner, Lithocolletis ringoniella MATSUMURA (Lepidoptera: Gracilariidae) [O] . Takeshi UJIYE 1972

机译：苹果叶矿工的交配行为研究，Lithocolletis Ringoniella Matsumura（Lepidoptera：Gracilariidae）

1. 苹果蠹蛾β-actin基因cDNA的克隆、序列分析及mRNA表达稳定性检测 [J] . 杨雪清 ,张雅林 . 西北农林科技大学学报（自然科学版） . 2014,第011期

2. 小菜蛾几丁质酶基因的克隆及表达分析 [J] . 申建梅 ,陈炳翰 ,黄丹青 . 环境昆虫学报 . 2018,第001期

3. 米蛾β-actin基因cDNA片段的克隆及表达量检测 [J] . 丛斌 ,张明珠 ,胡志凤 . 沈阳农业大学学报 . 2016,第001期

4. 小菜蛾肌球蛋白重链部分cDNA序列克隆、表达概况及分析 [J] . 龚亮 ,钟国华 ,任珍珍 . 江西农业大学学报 . 2010,第001期

5. 苹果蠹蛾几丁质脱乙酰基酶2的基因克隆、表达模式和分子特性 [J] . 石国良 ,武强 ,杨念婉 . 中国农业科学 . 2021,第010期

6. 抗性小菜蛾钠离子通道cDNA片段的克隆 [C] . 王建军 ,韩召军 . 中国昆虫学会2000年学术年会 . 2000

7. 金纹细蛾几丁质酶基因的克隆及原核表达 [A] . 李瑶 . 2012

1. 一种含金纹细蛾几丁质酶基因重组质粒的构建和应用 [P] . 中国专利： CN102517308A . 2012-06-27

2. 降低了高表达基因来源的cDNA克隆含有率的cDNA文库的制备方法 [P] . 中国专利： CN102124106B . 2014.06.25

3. Method for preparation of (4Ｅ, 10Ｚ)-tetradecadienylacetate as major sex pheromone of apple leaf miner moth, Phyllonorycter ringoniella [P] . 外国专利： KR101530004B1 . 2015-06-19

机译：（4E，10Ｚ）-十四碳二烯基乙酸酯作为苹果叶蛾的主要性信息素的制备方法

4. METHOD FOR PREPARATION OF (4E, 10Z)-TETRADECADIENYLACETATE AS MAJOR SEX PHEROMONE OF APPLE LEAF MINER MOTH [P] . 外国专利： KR20150024991A . 2015-03-10

机译：苹果叶蛾主要性信息素的（4E，10Z）-四癸二烯酸酯的制备方法

5. USE OF TETRAMIC ACID DERIVATIVES FOR CONTROLLING INSECTS FROM THE ORDER BEETLES (COLEOPTERA), THRIPS (TYSANOPTERA), BUGS (HEMIPTERA), FLIES (DIPTERA), LEAFHOPPERS (AUCHENORRHYNCHA) AND THE FAMILIES GALL MIDGES (CECIDOMYIIDAE), LEAF MINERS (GRACILLARIIDAE), TORTRIX MOTHS (TORTRICIDAE) AND SAWFLIES (TENTHREDINIDAE) [P] . 外国专利： CA2651729A1 . 2007-11-22

机译：使用四乙酸衍生物来控制甲虫，甲虫，TY虫，半翅目，蝇类（双翅目），叶足类（足纲）和粉虱（蛛网状），昆虫科TOR蛾（TOR螨）和锯齿（（TEN螨）

相关主题

Cloning and functional expression of a chitinase cDNA from the apple leaf miner moth Lithocolletis ringoniella

摘要

著录项

相似文献

相关主题

期刊订阅