Loop-loop interaction of HIV-1 TAR RNA with N3' right-arrow P5' deoxyphosphoramidate aptamers inhibits in vitro Tat-mediated transcription.

Toulme JJ

首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Loop-loop interaction of HIV-1 TAR RNA with N3' right-arrow P5' deoxyphosphoramidate aptamers inhibits in vitro Tat-mediated transcription.

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Loop-loop interaction of HIV-1 TAR RNA with N3' right-arrow P5' deoxyphosphoramidate aptamers inhibits in vitro Tat-mediated transcription.

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摘要

A hairpin RNA aptamer has been identified by in vitro selection against the transactivation-responsive element (TAR) of HIV-1. A nuclease-resistant N3' --> P5' phosphoramidate isosequential analog of this aptamer also folds as a hairpin and forms with TAR a loop-loop kissing demonstrated by electrophoretic mobility-shift assays and surface plasmon resonance experiments. The key structural determinants, which contribute to the stability of the RNA aptamer-TAR complex, loop complementarity and the GA residues closing the aptamer loop, remain crucial for the N3' --> P5' aptamer-TAR complex. Moreover, the N3' --> P5' phosphoramidate aptamer specifically interferes with the binding of a peptide derived from the transactivator protein (Tat) peptide to TAR and selectively inhibits the Tat-mediated transcription in an in vitro assay, which marks this nuclease-resistant aptamer as a relevant candidate for experiments in cells.

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《Proceedings of the National Academy of Sciences of the United States of America》 |2002年第15期|9709-9714|共6页
作者
Toulme JJ;
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作者单位

Institut National de la Sante et de la Recherche Medicale U386, Universite Victor Segalen, 33076 Bordeaux Cedex, France;

Institut Europeen de Chimie et Biologie, 16 Avenue Pey Berland, 33607 Pessac, France;

Medical Research Council, Laboratory of Mol;

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收录信息美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
原文格式 PDF
正文语种英语
中图分类自然科学总论;
关键词
aptamer; RNA; In Vitro; HIV-1; phosphoramidate;

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1. RNA and N3'--P5' kissing aptamers targeted to the trans-activation responsive (TAR) RNA of the human immunodeficiency virus-1. [J] . Darfeuille F, Cazenave C, Gryaznov SDuconge FDi-Primo CToulme JJ Nucleosides, nucleotides and nucleic acids . 2001,第7期

机译：RNA and N3'--P5' kissing aptamers targeted to the trans-activation responsive (TAR) RNA of the human immunodeficiency virus-1.
2. Structural RNA mimetics: N3'-->P5' phosphoramidate DNA analogs of HIV-1 RRE and TAR RNA form A-type helices that bind specifically to Rev and Tat-related peptides. [J] . Rigl CT, Lloyd DH, Tsou DS, Biochemistry . 1997,第3期

机译：结构RNA模拟物：HIV-1 RRE和TAR RNA的N3'-> P5'氨基磷酸DNA类似物形成A型螺旋，可特异性结合Rev和Tat相关肽。
3. RNA and N3'-->P5' kissing aptamers targeted to the trans-activation responsive (TAR) RNA of the human immunodeficiency virus-1. [J] . Darfeuille F, Cazenave C, Gryaznov S, Nucleosides, nucleotides and nucleic acids . 2001,第7期

机译：RNA和N3'-> P5'吻合适体，靶向人类免疫缺陷病毒1的反式激活应答（TAR）RNA。
4. Loop–loop interaction of HIV-1 TAR RNA with N3′ → P5′ deoxyphosphoramidate aptamers inhibits in vitro Tat-mediated transcription [O] . Fabien Darfeuille, Andrey Arzumanov, Sergei Gryaznov, 2002

机译：HIV-1 TAR RNA与N3→P5脱氧磷酰胺酸适体的环-环相互作用抑制Tat介导的体外转录
5. Loop–loop interaction of HIV-1 TAR RNA with N3′ → P5′ deoxyphosphoramidate aptamers inhibits in vitro Tat-mediated transcription [O] . Darfeuille, Fabien, Arzumanov, Andrey, Gryaznov, Sergei, 2002

机译：HIV-1 TAR RNA与N3'→P5'脱氧磷酰胺酸适体的环-环相互作用抑制Tat介导的体外转录

Loop-loop interaction of HIV-1 TAR RNA with N3' right-arrow P5' deoxyphosphoramidate aptamers inhibits in vitro Tat-mediated transcription.

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