On the Mechanism of Interaction of Organic Solvents with the Active Site of alpha-Chymotrypsin

E. A. Belyaeva; D. V. Gra; N. L. Eremeev

首页> 外文期刊>Biochemistry >On the Mechanism of Interaction of Organic Solvents with the Active Site of alpha-Chymotrypsin

【24h】

On the Mechanism of Interaction of Organic Solvents with the Active Site of alpha-Chymotrypsin

机译：有机溶剂与α-胰凝乳蛋白酶活性位点相互作用的机理

获取原文

获取原文并翻译 | 示例

掌桥外文数据库（机构版） >>

开具论文收录证明 >>

文献代查 >>

页面导航

摘要
著录项
相似文献
相关主题

摘要

Kinetic behavior of alpha-chymotrypsin in the reaction of hydrolysis of the N-acetyl-L-tyrosine derivatives was investigated in non-denaturing water-dimethylsulfoxide and water-ethanol mixtures. Similar specific interactions between the two solvents and the active site of alpha-chymotrypsin were shown to result in similar kinetic effects. It is proposed that the changes in the active site structure of the enzyme caused by the interaction with the organic solvents ("conformational isomer" formation) resulted in two parallel processes-acceleration of the acyl-enzyme formation step and a decrease in the deacylation rate. The possible molecular mechanism of this phenomenon and an adequate kinetic model describing the data are discussed.

机译：在非变性水-二甲基亚砜和水-乙醇混合物中研究了α-胰凝乳蛋白酶在N-乙酰基-L-酪氨酸衍生物水解反应中的动力学行为。两种溶剂与α-胰凝乳蛋白酶的活性位点之间的相似特异性相互作用显示出相似的动力学效应。提出由与有机溶剂的相互作用引起的酶活性位点结构的改变（“构象异构体”的形成）导致两个平行的过程-酰基-酶形成步骤的加速和脱酰率的降低。。讨论了该现象的可能分子机理和描述数据的适当动力学模型。

著录项

来源
《Biochemistry》 |2002年第9期|共5页
作者
E. A. Belyaeva; D. V. Gra; N. L. Eremeev;
展开▼
作者单位

展开▼
收录信息
原文格式 PDF
正文语种 eng
中图分类生物化学;
关键词
alpha-chymotrypsin; dimethylsulfoxide; ethanol; mechanism of action;

机译：α-胰凝乳蛋白酶;二甲亚砜;乙醇;作用机理;

相似文献

外文文献
中文文献
专利

1. On the Mechanism of Interaction of Organic Solvents with the Active Site of alpha-Chymotrypsin [J] . E. A. Belyaeva, D. V. Gra, N. L. Eremeev Biochemistry . 2002,第9期

机译：有机溶剂与α-胰凝乳蛋白酶活性位点相互作用的机理
2. Solvent dependence of Stokes shift for organic solute-solvent systems: A comparative study by spectroscopy and reference interaction-site model-self-consistent-field theory [J] . Nishiyama K., Watanabe Y., Yoshida N., The Journal of Chemical Physics . 2013,第9期

机译：斯托克斯位移对有机溶质-溶剂体系的溶剂依赖性：光谱学和参考相互作用-位点-模型-自洽场理论的比较研究
3. Solvent dependence of Stokes shift for organic solute-solvent systems: A comparative study by spectroscopy and reference interaction-site model-self-consistent-field theory [J] . Nishiyama K., Watanabe Y., Yoshida N., The Journal of Chemical Physics . 2013,第9期

机译：斯托克斯位移对有机溶质-溶剂体系的溶剂依赖性：光谱学和参考相互作用-位点-模型-自洽场理论的比较研究
4. Computational Simulations of the Interactions between Acetyl-Coenzyme-A Carboxylase and Clodinafop: Resistance Mechanism Due to Active and Nonactive Site Mutations [C] . Xiao-Lei Zhu, Hao Ge-Fei, Chang-Guo Zhan, ICMSI;International conference of molecular simulations and applied informatics technologies . 2010

机译：乙酰辅酶A羧化酶与氯丁那普之间相互作用的计算模拟：由于主动和非主动位点突变的抵抗机制。
5. Exploring the mechanisms of redox potential tuning and substrate/active-site interactions in iron- and manganese-bound superoxide dismutases and synthetic analogues. [D] . Gutman, Craig T. 2013

机译：探索与铁和锰结合的超氧化物歧化酶和合成类似物中氧化还原电位调节和底物/活性位点相互作用的机理。
6. The active site and mechanism of action of bovine pancreatic ribonuclease. 4. The activity in inert organic solvents and alcohols [O] . Dorothy Findlay, A. P. Mathias, B. R. Rabin 1962

机译：牛胰核糖核酸酶的活性位点和作用机理。 4.在惰性有机溶剂和醇中的活性
7. The active site and mechanism of action of bovine pancreatic ribonuclease. 4. The activity in inert organic solvents and alcohols [O] . Findlay, Dorothy, Mathias, A. P., Rabin, B. R. 1962

机译：牛胰核糖核酸酶的活性位点和作用机理。 4.在惰性有机溶剂和醇中的活性

On the Mechanism of Interaction of Organic Solvents with the Active Site of alpha-Chymotrypsin

摘要

著录项

相似文献

相关主题

期刊订阅