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首页> 外文期刊>Canadian Journal of Plant Science >Somatic hybridization between Solanum tuberosum and S. cardiophyllum
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Somatic hybridization between Solanum tuberosum and S. cardiophyllum

机译：马铃薯与心叶葡萄球菌的体细胞杂交

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摘要

The wild diploid Mexican species, Solanum cardiophyllum Lindl. (2n = 2x = 24), is resistant to important potato diseases. However, introgression of resistance to the tetraploid cultivated potato (S. tuberosum L.) (2n = 4x = 48) by conventional crossing is not feasible due to the difference in their endosperm balance number between these species. Somatic hybrids between S. cardiophyllum and S. tuberosum were produced for the first time by electrofusion of protoplasts isolated from young leaves of eachparental line. The hybrid nature of the regenerated plants was confirmed based on morphology, chromosome number and DNA species-specific RAPD markers. All the somatic hybrids produced a violet pigmentation on their stems and petioles, which resembled the wild partner. Most of the hybrid plants had 2n = 72 chromosomes and exhibited a morphology intermediate between the two fusion parents, but with a tendency towards cultivated potato. These plants flowered and set fruit when backcrossed with their S. tuberosum fusion parent.
机译：野生的二倍体墨西哥物种，茄属心叶茄。 （2n = 2x = 24），可以抵抗重要的马铃薯疾病。但是，由于常规杂交对这些四倍体栽培马铃薯（S. tuberosum L.）（2n = 4x = 48）的抗性渗入是不可行的，因为它们之间的胚乳平衡数不同。通过电融合从每个亲本系的幼叶中分离出的原生质体，首次产生了S. cardiophyllum和S. tuberosum之间的体细胞杂种。根据形态，染色体数目和DNA物种特异性的RAPD标记确定了再生植物的杂种特性。所有的体细胞杂种在其茎和叶柄上都产生了紫色的色素沉着，类似于野生伴侣。大多数杂种植物具有2n = 72个染色体，并且在两个融合亲本之间表现出一种形态学中间产物，但是倾向于种植马铃薯。这些植物与马铃薯的融合链亲本回交后开花并结实。

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