...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Biochemistry >IN VIVO PHOSPHORYLATION OF HISTONE H1 VARIANTS DURING THE CELL CYCLE
【24h】

IN VIVO PHOSPHORYLATION OF HISTONE H1 VARIANTS DURING THE CELL CYCLE

机译：细胞周期中组蛋白H1变种的体内磷酸化

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

In vivo phosphorylation of the five histone H1 variants H1a-H1e including H1(0) in NIH 3T3 mouse fibroblasts was examined during the eel cycle by using a combination of HPLC techniques and conventional AU gel electrophoresis. Phosphorylation starts during the late G1 phase and increases throughout the S phase. In the late S phase, the H1 variants exist as a combination of molecules containing 0 or 1 (H1a, H1c), 0-2 (H1d), or 0-3 (H1b, H1e) phosphate groups with a share of unphosphorylated protein ranging between 35% and 75%, according to the particular subtype. Pulse-chase experiments show that phosphorylation during the S phase is a dynamic phosphorylation process with a limited phosphorylation maximum. In most H1 subtypes, phosphorylation occurs very rapidly at the G2/M transition with only small amounts of intermediate phosphorylated molecules. Phosphorylation of mouse H1c, however, occurs stepwise during this transition. Phosphorylated mouse histone subtypes from cells in mitosis contain four phosphate groups in the case of H1a, H1c, and H1e and five in the case of H1b and H1d. Comparison of the mouse phosphorylation pattern to that in rat C-6 glioma cells showed differences for H1e and H1d. By comparing the different phosphorylation patterns of the individual H1 variants during the cell cycle, we were able to classify the H1 histones into subtypes with low (H1a, H1c, H1(0)) and high (H1b, H1d, H1e) phosphorylation levels.
机译：通过结合使用HPLC技术和常规AU凝胶电泳，在鳗鱼周期中检查了NIH 3T3小鼠成纤维细胞中包括H1（0）在内的五个组蛋白H1变体H1a-H1e的体内磷酸化。磷酸化在G1晚期开始，并在整个S阶段增加。在S期后期，H1变体以包含0或1（H1a，H1c），0-2（H1d）或0-3（H1b，H1e）磷酸基团的分子的组合形式存在，并且部分非磷酸化蛋白根据特定的亚型，介于35％和75％之间。脉冲追踪实验表明，S期的磷酸化是一个动态的磷酸化过程，其磷酸化最大值受到限制。在大多数H1亚型中，只有少量的中间磷酸化分子会在G2 / M过渡处非常迅速地发生磷酸化。但是，在此过渡过程中，小鼠H1c的磷酸化会逐步发生。来自H1a，H1c和H1e的有丝分裂细胞的磷酸化小鼠组蛋白亚型包含四个磷酸基，而对于H1b和H1d则包含五个磷酸基团。小鼠磷酸化模式与大鼠C-6胶质瘤细胞的磷酸化模式的比较显示，H1e和H1d有所不同。通过比较细胞周期中各个H1变体的不同磷酸化模式，我们能够将H1组蛋白分为具有低（H1a，H1c，H1（0））和高（H1b，H1d，H1e）磷酸化水平的亚型。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号