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首页> 外文期刊>Matrix biology: Journal of the International Society for Matrix Biology >The major basement membrane components localize to the chondrocyte pericellular matrix - A cartilage basement membrane equivalent?
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The major basement membrane components localize to the chondrocyte pericellular matrix - A cartilage basement membrane equivalent?

机译：基底膜的主要成分位于软骨细胞周细胞基质-软骨基底膜的等效物？

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摘要

In this study, we demonstrate that articular cartilage chondrocytes are surrounded by the defining basement membrane proteins laminin, collagen type IV, nidogen and perlecan, and suggest that these form the functional equivalent of a basement membrane. We found by real-time PCR that mouse chondrocytes express these four cardinal components of basement membranes and demonstrated by immunohistochemistry that the proteins are present in bovine and mouse cartilage tissues and are deposited in a thin pericellular structure. Immunoelectron microscopy confirmed high laminin concentration in the pericellular matrix. In cartilage from newborn mice, basement membrane components are widespread in the territorial and interterritorial matrix, while in mature cartilage of adult mice the basement membrane components are localized mainly to a narrow pericellular zone. With progression into old age, this layer becomes less distinct, especially in areas of obvious mechanical attrition. Interestingly, individual laminin subunits were located in different zones of the cartilage, with laminin alpha1 showing preferential localization around a select population of superficial layer chondrocytes. We propose that the chondrocyte, like several other cell types of mesenchymal origin, is surrounded by the functional equivalent of a basement membrane. This structure is presumably involved in maintaining chondrocyte phenotype and viability and may well allow a new understanding of cartilage development and provide clues to the progression of degenerative joint disorders.
机译：在这项研究中，我们证明了关节软骨软骨细胞被定义为基底膜蛋白层粘连蛋白，IV型胶原蛋白，胶原蛋白和全白蛋白包围，并暗示它们形成了基底膜的功能等同物。我们通过实时PCR发现，小鼠软骨细胞表达了基底膜的这四个主要组成部分，并通过免疫组织化学证明了该蛋白存在于牛和小鼠软骨组织中，并沉积在一个薄的细胞周围结构中。免疫电子显微镜证实在细胞周围基质中层粘连蛋白浓度高。在新生小鼠的软骨中，基底膜成分广泛分布于领土和疆域基质中，而在成年小鼠的成熟软骨中，基底膜成分主要定位于狭窄的细胞周围区域。随着年龄的增长，这一层变得越来越不明显，特别是在机械磨损明显的区域。有趣的是，单个层粘连蛋白亚基位于软骨的不同区域，层粘连蛋白α1显示在表层软骨细胞的选定群体周围优先定位。我们建议软骨细胞，像其他几种间充质来源的细胞类型一样，被基底膜的功能等同物包围。据推测，该结构参与维持软骨细胞表型和生存力，并且可能很好地使人们对软骨的发展有了新的认识，并为退行性关节疾病的进展提供了线索。

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