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首页> 外文期刊>Matrix biology: Journal of the International Society for Matrix Biology >Gene expression profiling of differentiating embryonic stem cells expressing dominant negative fibroblast growth factor receptor 2.
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Gene expression profiling of differentiating embryonic stem cells expressing dominant negative fibroblast growth factor receptor 2.

机译：表达显性负成纤维细胞生长因子受体2的分化胚胎干细胞的基因表达谱

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摘要

Embryonic stem (ES) cells are derived from the inner cell mass of the blastocyst and can be cultured as three-dimensional embryoid bodies (EBs) in which embryonic pregastrulation stages are faithfully mimicked. Fibroblast growth factor receptors (mainly FGFR2) are involved in the first differentiation events during early mammalian embryogenesis. It has been demonstrated that the presence of FGFR2 is a prerequisite for laminin-111 and collagen type IV synthesis and subsequently basement membrane formation in EBs. To identify genes that are influenced by FGFR signalling, we performed global gene expression profiling of differentiating EBs expressing dominant negative FGFR2 (dnFGFR2), acquiring an extensive catalogue of down- and up-regulated genes. We show a strong down-regulation of endodermal and basement membrane related genes, which strengthen the view that the FGFR signalling pathway is a main stimulator of basement membrane synthesis in EBs. We further present down-regulation of genes previously not linked to FGFR signalling, and in addition an active transcription of some mesodermal related genes in differentiating dnFGFR2 EBs.
机译：胚胎干（ES）细胞源自胚泡的内部细胞团，可以培养为忠实模仿胚胎预妊娠阶段的三维胚状体（EB）。成纤维细胞生长因子受体（主要是FGFR2）参与早期哺乳动物胚胎发生过程中的第一个分化过程。已经证明，FGFR2的存在是层粘连蛋白111和IV型胶原合成以及随后在EB中形成基底膜的先决条件。为了鉴定受FGFR信号影响的基因，我们对表达显性负FGFR2（dnFGFR2）的分化EB进行了全局基因表达谱分析，获得了下调和上调基因的广泛目录。我们显示出强烈的内胚层和基底膜相关基因的下调，这增强了FGFR信号通路是EBs基底膜合成的主要刺激因素的观点。我们进一步介绍了以前与FGFR信号不相关的基因的下调，以及在区分dnFGFR2 EB时一些中胚层相关基因的主动转录。

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