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首页> 外文期刊>Matrix biology: Journal of the International Society for Matrix Biology >Codistribution analysis of elastin and related fibrillar proteins in early vertebrate development.
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Codistribution analysis of elastin and related fibrillar proteins in early vertebrate development.

机译：弹性蛋白和相关纤维蛋白在脊椎动物早期发育中的共分布分析。

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摘要

Elastin is an extracellular matrix protein found in adult and neonatal vasculature, lung, skin and connective tissue. It is secreted as tropoelastin, a soluble protein that is cross-linked in the tissue space to form an insoluble elastin matrix. Cross-linked elastin can be found in association with several microfibril-associated proteins including fibrillin-1, fibrillin-2 and fibulin-1 suggesting that these proteins contribute to elastic fiber assembly, structure or function. To date, the earliest reported elastin expression was in the conotruncal region of the developing avian heart at 3.5 days of gestation. Here we report that elastin expression begins at significantly earlier developmental stages. Using a novel immunolabeling method, the deposition of elastin, fibrillin-1 and -2 and fibulin-1 was analyzed in avian embryos at several time points during the first 2 days of development. Elastin was found at the midline associated with axial structures such as the notochord and somites at 23 h of development. Fibrillin-1 and -2 and fibulin-1 were also expressed at the embryonic midline at this stage with fibrillin-1 and fibulin-1 showing a high degree of colocalization with elastin in fibers surrounding midline structures. The expression of these genes was confirmed by conventional immunoblotting and mRNA detection methods. Our results demonstrate that elastin polypeptide deposition occurs much earlier than was previously appreciated. Furthermore, the results suggest that elastin deposition at the early embryonic midline is accompanied by the deposition and organization of a number of extracellular matrix polypeptides. These filamentous extracellular matrix structures may act to transduce or otherwise stabilize dynamic forces generated during embryogenesis.
机译：弹性蛋白是一种在成人和新生儿脉管系统，肺，皮肤和结缔组织中发现的细胞外基质蛋白。它以原弹性蛋白的形式分泌，这是一种可溶性蛋白，在组织空间中交联形成不溶的弹性蛋白基质。可以发现交联的弹性蛋白与几种与微纤维相关的蛋白（包括原纤维蛋白-1，原纤维蛋白-2和原纤维蛋白-1）相关联，表明这些蛋白有助于弹性纤维的组装，结构或功能。迄今为止，据报道最早的弹性蛋白表达是在妊娠3.5天时在发育中的禽心脏的锥孔区域。在这里，我们报告弹性蛋白表达始于明显的早期发展阶段。使用一种新型的免疫标记方法，在发育的前两天中的几个时间点，分析了禽胚胎中弹性蛋白，原纤维蛋白-1和-2和纤维蛋白-1的沉积。在发育的23小时内，在与中轴和脊索等轴向结构相关的中线发现了弹性蛋白。 Fibrillin-1和-2和fibulin-1在此阶段也在胚胎中线表达，而fibrillin-1和fibulin-1在中线结构周围的纤维中显示出与弹性蛋白的高度共定位。这些基因的表达通过常规的免疫印迹和mRNA检测方法得到证实。我们的结果表明，弹性蛋白多肽的沉积发生的时间比以前认识的要早得多。此外，结果表明，弹性蛋白在早期胚胎中线的沉积伴随着许多细胞外基质多肽的沉积和组织。这些丝状细胞外基质结构可用于转导或稳定胚胎发生过程中产生的动力。

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