• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Matrix biology: Journal of the International Society for Matrix Biology >Characterization of the human type XVIII collagen gene and proteolytic processing and tissue location of the variant containing a frizzled motif.
【24h】

Characterization of the human type XVIII collagen gene and proteolytic processing and tissue location of the variant containing a frizzled motif.

机译：人型XVIII胶原基因的特征以及含有卷曲基序的变体的蛋白水解过程和组织位置。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Human type XVIII collagen was found to be expressed as three variants, termed NC1-303, NC1-493 and NC1-728, differing in their N-terminal non-collagenous domains (NC1). The corresponding gene was found to be approximately 105 kb in size and contain 43 exons. The short variant is derived from utilization of an upstream promoter associated with the first two exons of the gene. The two other variants are derived from a downstream promoter and alternative splicing of exon 3, resulting in 192 residues of shared sequences characterized by a putative approximately 30 residue conserved coiled-coil motif and 235 residues of sequences specific to NC1-728. The NC1-728 variant has a conserved cysteine-rich domain homologous with the ligand-binding part of the frizzled proteins. A polyclonal antibody specific to the NC1-728 variant was generated, and immunostaining of fetal tissues revealed staining in lung and skeletal muscle. Human serum contained 173- and 144-kDa alpha1(XVIII) chains corresponding to the NC1-728and NC1-493 variants, respectively. A 200-kDa polypeptide was detected in cells transfected with a cDNA construct corresponding to the full-length NC1-728 variant, and EBNA-293 cells endogenously synthesizing low amounts of type XVIII collagen had a 45-kDa fragment in their culture medium that corresponded to most of the NC1 domain of the NC1-728 variant, suggesting processing of the N-terminal frizzled-containing domain.
机译：发现人类XVIII型胶原蛋白表达为三种变体，分别称为NC1-303，NC1-493和NC1-728，其N端非胶原结构域（NC1）不同。发现相应的基因大小约为105 kb，包含43个外显子。该短变体源自与该基因的前两个外显子相关的上游启动子的利用。其他两个变体衍生自下游启动子和外显子3的可变剪接，导致共有序列的192个残基，其特征是假定的约30个残基保守的卷曲螺旋基序和235个对NC1-728特异的序列残基。 NC1-728变体具有与卷曲蛋白的配体结合部分同源的保守的富含半胱氨酸的结构域。产生了对NC1-728变体特异的多克隆抗体，胎儿组织的免疫染色揭示了肺和骨骼肌的染色。人血清包含分别对应于NC1-728和NC1-493变体的173-kDa和144-kDa alpha1（XVIII）链。在用对应于全长NC1-728变体的cDNA构建体转染的细胞中检测到200 kDa的多肽，内源性合成少量XVIII型胶原的EBNA-293细胞在其培养基中具有一个45 kDa的片段，对应于对于NC1-728变体的大多数NC1结构域，提示对N端含卷曲结构域的加工。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号