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首页> 外文期刊>Matrix biology: Journal of the International Society for Matrix Biology >Perlecan and its immunoglobulin like domain IV are abundant in vitreous and serum of the chick embryo.
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Perlecan and its immunoglobulin like domain IV are abundant in vitreous and serum of the chick embryo.

机译：Perlecan及其免疫球蛋白样结构域IV在雏鸡的玻璃体和血清中含量丰富。

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摘要

Perlecan is a highly conserved heparan sulfate proteoglycan in cartilage and basement membranes. We identified chick perlecan and a 90 KD perlecan fragment in vivo using a newly generated monoclonal antibody. Chick perlecan is, like its human and mouse homologue, a hybrid heparan sulfate/chondroitin sulfate proteoglycan with a core protein of 400 KD. Analysis of the 90 KD fragment by Matrix Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry (MALDI-TOF MS) and Capillary LC nano Electrospray Ionization tandem MS (LC nano ESI MS/MS) showed that it belonged to domain IV of the perlecan core protein. We found that full-length perlecan and its domain IV fragment are abundant in embryonic vitreous body and serum. Their expression in vitreous and serum is greatly down-regulated shortly after hatching of the chick. We speculate that the abundance of perlecan in the embryonic circulation and vitreous reflects the ongoing formation of new BMs in the expanding vascular system and the growing retina. In addition, we found that perlecan as a substrate does not support, rather inhibits neurite outgrowth.
机译：Perlecan是软骨和基底膜中高度保守的硫酸乙酰肝素蛋白聚糖。我们使用一种新生成的单克隆抗体在体内鉴定了小鸡珍珠雀和一个90 KD珍珠雀片段。像其人和小鼠的同系物一样，小鸡Perlecan是具有400 KD核心蛋白的杂种硫酸乙酰肝素/硫酸软骨素蛋白聚糖。通过基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）和毛细管LC纳米电喷雾串联电离质谱（LC nano ESI MS / MS）分析90 KD片段，发现该片段属于Perlecan的IV区核心蛋白。我们发现全长珍珠白及其结构域IV片段在胚胎玻璃体和血清中含量丰富。孵化后不久，它们在玻璃体和血清中的表达大大下调。我们推测，在胚胎循环和玻璃体中丰富的珍珠岩反映出不断扩大的血管系统和不断增长的视网膜中新的BM的持续形成。另外，我们发现，作为基质的全白蛋白聚糖不支持而是抑制神经突的生长。

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