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首页> 外文期刊>Biochemistry >Wheat germ poly(A) binding protein enhances the binding affinity of eukaryotic initiation factor 4F and (iso)4F for cap analogues
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Wheat germ poly(A) binding protein enhances the binding affinity of eukaryotic initiation factor 4F and (iso)4F for cap analogues

机译：小麦胚芽poly（A）结合蛋白增强了真核起始因子4F和（iso）4F对帽类似物的结合亲和力

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摘要

Most eukaryotic mRNAs contain a 5' cap (m(7)GpppX) and a 3' poly(A) tail to increase synergistically the translational efficiency. Recently, the poly(A) binding protein (PABP) and cap-binding protein, eIF-4F, were found to interact [Le et al, (1997) J. Biol, Chern. 272, 16247-16255; Tarun and Sachs (1996) EMBO J. 15, 7168-7177], These data suggest that PABP may exert its effect on translational efficiency either by increasing the formation of initiation factor-mRNA complex or by enhancing ribosome recycling. To investigate the functional consequences of these interactions, the fluorescent cap analogue ant-m(7)GTP, which is an environmentally sensitive fluorescent probe [Ren and Goss (1996) Nucleic Acids Res. 24, 3629-3634] was used to investigate the cap-binding affinity. Our data show that the binding of eIF-(iso)4F or eIF-4F to cap analogue enhanced their binding affinity toward PABP approximately 40-fold. Similarly, the eIF-4F/PABP or eIF-(iso)4F/PABP complexes show a 40-fold enhancement of cap analogue binding as compared to eIF-4F or eIF-(iso)4F alone, At least part of the enhancement of the translational initiation by PABP can be accounted for by direct changes in cap-binding affinity, The interactions of these components also suggest a mechanism whereby the poly(A) tail is brought into close proximity with m(7)G cap, This effect was examined by fluorescence energy transfer, and it was determined that the PABP/eIF-4F complex could bind both poly(A) and 5' cap simultaneously. [References: 30]
机译：大多数真核mRNA包含一个5'帽（m（7）GpppX）和一个3'poly（A）尾巴，以协同提高翻译效率。最近，发现聚（A）结合蛋白（PABP）和帽结合蛋白eIF-4F相互作用[Le等人，（1997）J.Biol，Chern.Biol.Chem.Soc.Sci。，Vol.5，No.1，1992，J.Biol.Chem。，1997。 272，16247-16255; Tarun and Sachs（1996）EMBO J. 15，7168-7177]，这些数据表明，PABP可能通过增加起始因子-mRNA复合物的形成或通过增强核糖体再循环来发挥其对翻译效率的作用。为了研究这些相互作用的功能后果，荧光帽类似物ant-m（7）GTP是一种对环境敏感的荧光探针[Ren and Goss（1996）Nucleic Acids Res。 24，3629-3634]用于研究帽结合亲和力。我们的数据表明，eIF-（iso）4F或eIF-4F与帽类似物的结合增强了其对PABP的结合亲和力约40倍。类似地，与单独的eIF-4F或eIF-（iso）4F相比，eIF-4F / PABP或eIF-（iso）4F / PABP复合物显示帽类似物结合增强40倍，至少部分增强PABP的翻译起始可以通过帽结合亲和力的直接变化来解释。这些成分之间的相互作用还表明了一种机制，使poly（A）尾部与m（7）G cap紧密接近，这种作用是通过荧光能量转移检测，确定PABP / eIF-4F复合物可以同时结合poly（A）和5'帽。 [参考：30]

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