Global structure and mechanical properties of a 10-bp nucleosome positioning motif.

Roychoudhury M; Sitlani A; Lapham JCrothers DM

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Global structure and mechanical properties of a 10-bp nucleosome positioning motif.

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The method of DNA cyclization kinetics reveals special properties of the TATAAACGCC sequence motif found in DNA sequences that have high affinity for core histones. Replacement of 30 bp of generic DNA by three 10-bp repeats of the motif in small cyclization constructs increases cyclization rates by two orders of magnitude. We document a 13 degrees bend in the motif and characterize the direction of curvature. The bending force constant is smaller by nearly 2-fold and there is a 35 decrease in the twist modulus, relative to generic DNA. These features are the likely source of the high affinity for bending around core histones to form nucleosomes. Our results establish a protocol for determination of the ensemble-averaged global solution structure and mechanical properties of any approximately 10-bp DNA sequence element of interest, providing information complementary to that from NMR and crystallographic structural studies.

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《Proceedings of the National Academy of Sciences of the United States of America》 |2000年第25期|13608-13613|共6页
作者
Roychoudhury M; Sitlani A; Lapham JCrothers DM;
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作者单位

Department of Chemistry, P.O. Box 208107, Yale University, New Haven, CT 06520, USA.;

sy.ucsd.edu;

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收录信息美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
原文格式 PDF
正文语种英语
中图分类自然科学总论;
关键词
Nucleic Acid Conformation; Nucleosomes; DNA Primers; 核酸构象; 核小体;

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Global structure and mechanical properties of a 10-bp nucleosome positioning motif.

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